Finogenova T V, Kamzolova S V, Dedyukhina E G, Shishkanova N V, Il'chenko A P, Morgunov I G, Chernyavskaya O G, Sokolov A P
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, p-t Nauki 5, Pushchino, Moscow region 142290, Russia.
Appl Microbiol Biotechnol. 2002 Aug;59(4-5):493-500. doi: 10.1007/s00253-002-1022-8. Epub 2002 Jun 22.
The effect of ethanol, zinc, and iron (Fe(2+) and Fe(3+)) concentration and of oxygen supply on cell growth and the production of citric acid (CA) and isocitric acid (ICA) from ethanol by mutant Yarrowia lipolytica N 1 was studied under continuous cultivation. The following peculiarities of Y. lipolytica metabolism were found: (1) intensive CA production occurred under yeast growth limitation by nitrogen; (2) inhibition of yeast growth by ethanol was accompanied by significant alterations in fatty acid composition of lipids; (3) the production of CA and ICA from ethanol required high concentrations of zinc and iron ions; (4) the intracellular iron concentration determined whether CA or ICA was predominantly formed; (5) the cell's requirement for oxygen depended on the intracellular iron concentration. The events taking place in the production of CA and ICA were evaluated through the activities of enzyme systems involved in the metabolism of ethanol and CA in this strain.
在连续培养条件下，研究了乙醇、锌、铁（Fe(2+)和Fe(3+)）浓度以及氧气供应对解脂耶氏酵母突变株N 1细胞生长以及由乙醇生产柠檬酸（CA）和异柠檬酸（ICA）的影响。发现解脂耶氏酵母代谢具有以下特点：（1）在氮限制酵母生长的条件下会大量产生CA；（2）乙醇对酵母生长的抑制伴随着脂质脂肪酸组成的显著变化；（3）由乙醇生产CA和ICA需要高浓度的锌离子和铁离子；（4）细胞内铁浓度决定了主要生成的是CA还是ICA；（5）细胞对氧气的需求取决于细胞内铁浓度。通过该菌株中参与乙醇和CA代谢的酶系统活性评估了CA和ICA生产过程中发生的事件。
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