Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Chełmońskiego Str. 37/41, 51-630, Wrocław, Poland,
Appl Biochem Biotechnol. 2014 Mar;172(6):3069-78. doi: 10.1007/s12010-014-0745-1. Epub 2014 Feb 1.
The aim of this study was to examine the impact of divalent copper, iron, manganese, and zinc ions on the production of erythritol from glycerol by Yarrowia lipolytica and their effect on the activity of erythrose reductase. No inhibitory effect of the examined minerals on yeast growth was observed in the study. Supplementation with MnSO4 · 7H2O (25 mg l(-1)) increased erythritol production by Y. lipolytica by 14.5%. In the bioreactor culture with manganese ion addition, 47.1 g l(-1) of erythritol was produced from 100.0 g l(-1) of glycerol, which corresponded to volumetric productivity of 0.87 g l(-1) h(-1). The addition of Mn(2+) enhanced the intracellular activity of erythrose reductase up to 24.9 U g(-1) of dry weight of biomass (DW), hence, about 1.3 times more than in the control.
本研究旨在考察二价铜、铁、锰和锌离子对解脂耶氏酵母利用甘油生产赤藓糖醇的影响，以及它们对赤藓糖还原酶活性的影响。研究中未观察到所检查的矿物质对酵母生长有抑制作用。补充 MnSO4·7H2O（25 mg l（-1））可使解脂耶氏酵母的赤藓糖醇产量提高 14.5%。在添加锰离子的生物反应器培养中，从 100.0 g l（-1）的甘油中生产了 47.1 g l（-1）的赤藓糖醇，对应的体积产率为 0.87 g l（-1）h（-1）。添加 Mn（2+）可使胞内赤藓糖还原酶活性提高至 24.9 U g（-1）干重生物质（DW），比对照提高了约 1.3 倍。
