Mantke R, Rocken C, Schubert D, Pross M, Sokolowski A, Halangk W, Lippert H, Schulz H-U
Department of General Surgery, Otto-von-Guericke-Universität Magdeburg, Leipziger Strasse 44, 39120 Magdeburg, Germany.
Langenbecks Arch Surg. 2002 Jul;387(3-4):170-6. doi: 10.1007/s00423-002-0297-7. Epub 2002 May 15.
Oxidative stress is a relevant event in the pathogenesis of acute pancreatitis. Investigations in vivo are limited because of the complexity of the organism and the short half-life of free radicals. The isolated perfused rat pancreas could be useful for investigations in the early phase of acute pancreatitis especially under conditions of oxidative stress.
External perfusions of the pancreatic glands of Wistar rats were carried out using a modified Krebs-Ringer buffer including an additive of the detergents Triton X-100 and a perfusion including hydrogen peroxide (0.0012%) or tert-butylhydroperoxide (0.0042%) or xanthine oxidase (0.1 U/ml). Changes in amylase, lipase, LDH in the portal outflow fluid and for histological alterations were analyzed.
Damage to pancreatic parenchyma using Triton X-100 was indicated by increased levels of pancreatic enzymes in the perfusion medium. During perfusion with hydrogen peroxide or tert-butylhydroperoxide we found no changes in pancreatic enzymes in the portal outflow. In contrast, perfusion with xanthine oxidase induced a significant elevation in lipase and amylase in the effusion fluid after 30 min. We found a significant increase in edema in the hydrogen peroxide and in the xanthine oxidase group. Focal necroses of the pancreatic parenchyma were detected in all groups of oxidative stress.
The isolated perfused rat pancreas is a valuable experimental model for investigating the early phase of pathophysiology in acute pancreatitis, for instance, the effect of oxidative stress as an early event in acute pancreatitis. Using hydrogen peroxide tert-butylhydroperoxide or xanthine oxidase, only xanthine oxidase was able to induce a typical elevation in the pancreas enzymes in the effusion fluid.
氧化应激是急性胰腺炎发病机制中的一个相关事件。由于生物体的复杂性和自由基的半衰期短，体内研究受到限制。分离灌注的大鼠胰腺可能有助于急性胰腺炎早期阶段的研究，尤其是在氧化应激条件下。
使用改良的 Krebs-Ringer 缓冲液对 Wistar 大鼠的胰腺进行体外灌注，该缓冲液包含去污剂 Triton X-100 添加剂，以及包含过氧化氢（0.0012%）或叔丁基过氧化氢（0.0042%）或黄嘌呤氧化酶（0.1 U/ml）的灌注液。分析门静脉流出液中淀粉酶、脂肪酶、乳酸脱氢酶的变化以及组织学改变。
灌注液中胰腺酶水平升高表明 Triton X-100 对胰腺实质造成了损伤。在用过氧化氢或叔丁基过氧化氢灌注期间，我们发现门静脉流出液中的胰腺酶没有变化。相比之下，用黄嘌呤氧化酶灌注 30 分钟后，渗出液中的脂肪酶和淀粉酶显著升高。我们发现过氧化氢组和黄嘌呤氧化酶组的水肿明显增加。在所有氧化应激组中均检测到胰腺实质的局灶性坏死。
分离灌注的大鼠胰腺是研究急性胰腺炎病理生理学早期阶段的有价值的实验模型，例如，氧化应激作为急性胰腺炎早期事件的作用。使用过氧化氢、叔丁基过氧化氢或黄嘌呤氧化酶，只有黄嘌呤氧化酶能够诱导渗出液中胰腺酶的典型升高。
