Kawamura Naoko, Sahara Ken
Biology Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido, Japan.
Dev Growth Differ. 2002 Aug;44(4):273-80. doi: 10.1046/j.1440-169x.2002.00641.x.
Bombyx spermatogonia are bipotential, producing nucleate eupyrene sperm and anucleate apyrene sperm. An in vitro cultivation of spermatocysts of Bombyx mori from spermatocytes to matured sperm was established. The present experiment made clear that: (i) spermatocysts must be isolated; (ii) constant shaking at 45 r.p.m. was necessary; and (iii) the addition of Bombyx hemolymph (BH) was indispensable for successful cultivation. In the absence of BH, spermatogenesis proceeded normally for 2 or 3 days and, thereafter, spermatocytes and sperm bundles began to degenerate. The best results for normal eupyrene spermatogenesis were obtained when culture medium containing BH of the corresponding stage was used in every exchange of the medium at 72 h intervals. None or only a small number of apyrene sperm bundles was produced by this culture system when spermatocysts from larval testes were used, although eupyrene spermatogenesis proceeded normally to form matured, or squeezed, sperm bundles.
家蚕精原细胞具有双潜能性，可产生有核的真核精子和无核的副核精子。建立了从精母细胞到成熟精子的家蚕精囊肿体外培养体系。本实验明确了：（i）必须分离精囊肿；（ii）以45转/分钟的速度持续振荡是必要的；（iii）添加家蚕血淋巴（BH）对于成功培养不可或缺。在没有BH的情况下，精子发生正常进行2或3天，此后，精母细胞和精子束开始退化。当每隔72小时更换培养基时使用含有相应阶段BH的培养基，可获得正常真核精子发生的最佳结果。当使用来自幼虫睾丸的精囊肿时，该培养系统产生的无核精子束很少或没有，尽管真核精子发生正常进行以形成成熟的或挤出的精子束。
