Elamaa Harri, Peterson Josi, Pihlajaniemi Taina, Destrée Olivier
Collagen Research Unit, Biocenter and Department of Medical Biochemistry, University of Oulu, P.O. Box 5000, Aapistie 7, 90014, Oulu, Finland.
Mech Dev. 2002 Jun;114(1-2):109-13. doi: 10.1016/s0925-4773(02)00014-x.
Xenopus laevis type XVIII collagen occurs in three variants, 22 + 1285 amino acid residues (signal peptide + mature protein), 23 + 1581 residues and 23 + 1886 residues in length, differing in their N-terminal non-collagenous domains. The region showing highest homology to mammalian counterparts is the C-terminal endostatin domain. All three variants are expressed, at different levels, during early and late stages of development, as demonstrated by reverse transcription-polymerase chain reaction. Whole-mount in situ hybridization shows that the short variant is expressed at high levels in the developing eye, the central nervous system, the otic vesicle, the head mesenchyme, the branchial arches and the pronephros, and at the boundaries between somites. The middle variant is expressed in the head mesenchyme, the branchial arches, the peripheral nervous system, the pronephros and the pronephric duct, and at the somite boundaries. The longest variant is weakly expressed in the head mesenchyme and branchial arches.
非洲爪蟾 XVIII 型胶原蛋白有三种变体，长度分别为 22 + 1285 个氨基酸残基（信号肽 + 成熟蛋白）、23 + 1581 个残基和 23 + 1886 个残基，其 N 端非胶原蛋白结构域不同。与哺乳动物对应物显示出最高同源性的区域是 C 端内皮抑素结构域。通过逆转录 - 聚合酶链反应证明，所有三种变体在发育的早期和晚期均有不同水平的表达。整体原位杂交显示，短变体在发育中的眼睛、中枢神经系统、耳泡、头部间充质、鳃弓和前肾以及体节之间的边界处高水平表达。中间变体在头部间充质、鳃弓、外周神经系统、前肾和前肾管以及体节边界处表达。最长的变体在头部间充质和鳃弓中弱表达。
