Wojciechowska Barbara, Pudelska Hanna
Institute of Plant Genetics, Polish Academy of Sciences, Poznań, Poland.
J Appl Genet. 2002;43(3):279-85.
Hybrids between Aegilops kotschyi and Ae. biuncialis with Secale cereale were synthesized. Five Ae. kotschyi and four Ae. biuncialis accessions, as well as one inbred and four self-compatible forms of Secale cereale were used for crossing. The hybrids were produced directly from cultured embryos or through embryo callus culture. Sixty hybrids, 11 involving Ae. kotschyi and 49 Ae. biuncialis, had a stable somatic chromosome number 2n = 3x = 21. The plants showed good vegetative vigour and tillering capacity. Morphologically the hybrids were intermediate between their parents and completely sterile. In vitro propagation of Ae. kotschyi and Ae. biuncialis x S. cereale hybrids revealed that their capacity for callus production and plantlet regeneration - varies.
合成了节节麦(Aegilops kotschyi)与二芒山羊草(Ae. biuncialis)和黑麦(Secale cereale)的杂种。使用了5份节节麦、4份二芒山羊草种质,以及1份黑麦自交系和4份自交亲和型黑麦进行杂交。杂种直接由培养的胚产生,或通过胚愈伤组织培养产生。60个杂种,其中11个涉及节节麦,49个涉及二芒山羊草,其体细胞染色体数稳定为2n = 3x = 21。这些植株表现出良好的营养活力和分蘖能力。杂种在形态上介于其亲本之间,并且完全不育。节节麦以及二芒山羊草×黑麦杂种的离体繁殖表明,它们产生愈伤组织和再生植株的能力各不相同。