Kalinowski Andrzej, Wojciechowska Barbara, Borzyszkowska Elzbieta
Institute of Plant Genetics, Polish Academy of Sciences, ul. Strzeszyńska 34, 60-479 Poznań, Poland.
J Appl Genet. 2003;44(1):35-43.
Seven enzymatic systems in F1 Aegilops kotschyi and Ae. biuncialis x Secale cereale hybrids, Aegilops kotschyi x S. cereale amphiploids and their parental species (Ae. kotschyi, Ae. biuncialis and S. cereale) were analysed by starch and polyacrylamide gel electrophoresis. Five of them (phosphoglucose isomerase, glutamic oxalacetic transaminase, esterase, acid phosphatase, and diaphorase) were polymorphic and two (malic dehydrogenase and superoxide dismutase) were monomorphic. Several isophorms of phosphoclucose isomerase, esterase, acid phosphatase, and diaphorase were detected in some hybrids and amphiploids, but absent in the parents. The role of regulators, translocations and recombination is discussed in relation to the origin of these new isophorms. Some parental isozymes were absent both in hybrids and amphiploids, probably as a result of the suppression of structural genes in new combinations of the three genomes.
通过淀粉凝胶电泳和聚丙烯酰胺凝胶电泳分析了F1代小伞山羊草、偏凸山羊草×黑麦杂种、小伞山羊草×黑麦双二倍体及其亲本物种(小伞山羊草、偏凸山羊草和黑麦)中的7种酶系统。其中5种(磷酸葡萄糖异构酶、谷草转氨酶、酯酶、酸性磷酸酶和黄递酶)具有多态性,2种(苹果酸脱氢酶和超氧化物歧化酶)为单态性。在一些杂种和双二倍体中检测到了磷酸葡萄糖异构酶、酯酶、酸性磷酸酶和黄递酶的几种同工酶,但在亲本中未检测到。讨论了调控因子、易位和重组在这些新同工酶起源中的作用。一些亲本同工酶在杂种和双二倍体中均未出现,这可能是由于三个基因组新组合中结构基因受到抑制的结果。