Jackson S, Ghali L, Harwood C, Storey A
Cancer Research UK, Skin Tumour Laboratory, Centre for Cutaneous Research, 2 Newark Street, London E1 2AT, UK.
Br J Cancer. 2002 Jul 29;87(3):319-23. doi: 10.1038/sj.bjc.6600431.
We have investigated the apoptotic levels and expression of the apoptotic inducer Bak in non-melanoma skin cancers. Squamous cell carcinomas of known human papillomavirus status from immunocompetent patients were analysed for the expression of the Bak protein, and the expression profile was compared both to the presence of apoptotic cells and the proliferation marker Ki-67. We demonstrate an inverse correlation between human papillomavirus positivity and Bak expression in squamous cell carcinomas, with concomitantly fewer apoptoic cells being detected in the human papillomavirus positive tumours. Bak expression was not observed in basal cell carcinomas irrespective of human papillomavirus status, suggesting that Bak only plays a role in signalling apoptosis in squamous, but not basal, cell cancers. No differences were observed in the proliferation rates between papillomavirus positive and negative squamous cell tumours. However, a significant decrease in the number of apoptotic cells was observed in human papillomavirus-positive squamous cell carcinomas which suggests that the virus may have significantly altered the relationship between proliferation and apoptosis in a proportion of these tumours.
我们研究了非黑色素瘤皮肤癌中凋亡水平及凋亡诱导因子Bak的表达情况。对免疫功能正常患者中已知人乳头瘤病毒状态的鳞状细胞癌进行分析,检测Bak蛋白的表达,并将其表达谱与凋亡细胞的存在情况及增殖标志物Ki-67进行比较。我们发现,在鳞状细胞癌中,人乳头瘤病毒阳性与Bak表达呈负相关,同时在人乳头瘤病毒阳性肿瘤中检测到的凋亡细胞较少。无论人乳头瘤病毒状态如何,在基底细胞癌中均未观察到Bak表达,这表明Bak仅在鳞状细胞癌而非基底细胞癌的凋亡信号传导中发挥作用。人乳头瘤病毒阳性和阴性鳞状细胞肿瘤之间的增殖率没有差异。然而,在人乳头瘤病毒阳性的鳞状细胞癌中观察到凋亡细胞数量显著减少,这表明该病毒可能在一定比例的这些肿瘤中显著改变了增殖与凋亡之间的关系。