Chang C H, Tsai R K, Chen G S, Yu H S, Chai C Y
Department of Dermatology, Kaohsiung Medical College, Taiwan, R.O.C.
J Cutan Pathol. 1998 Oct;25(9):457-62. doi: 10.1111/j.1600-0560.1998.tb01775.x.
To investigate the regulation of apoptosis and proliferation in arsenic-induced skin cancers, we examined the expression of bcl-2, p53, and Ki-67 using immunohistochemical staining. Thirty patients with Bowen's disease (BD), ten with basal cell carcinoma (BCC), eight with squamous cell carcinoma (SCC) and eleven of perilesional normal skin (PLN) of the non-sun exposure sites from endemic area were examined. The results showed that: 1) bcl-2 was expressed in all of the BCC homogeneously, in none of the SCC, and in 12/30 of the BD focally or homogeneously; 2) p53 was expressed in all of the arsenical skin cancers with a labelling index of 75 +/- 14% of BD, 50 +/- 17% of BCC, 61 +/- 15% of SCC, and also in all of the perilesional normal skin with a labelling index of 55 +/- 24%; 3) Ki-67 was expressed in all of the skin cancers with labelling index of 58 +/- 17% of BD, 12 +/- 7% of BCC, 47 +/- 21% of SCC, and in 9/11 of PLN with a labelling index of 41 +/- 24%. Expression of bcl-2 in BCC or BD is related to the phenotype of germinative basal cell. The constant expression of bcl-2 i early dysplastic cells of BD and the earliest expression of P53 in the basal cells of perilesional normal skin indicate that the initial step of arsenic-induced carcinogenesis is from the basal germinative cells. There is no mutual relationship between bcl-2, p53 or Ki-67 expression in any type of the arsenical skin cancers, but there is a positive correlation between p53 and Ki-67 expression identified in perilesional normal skin. BD had the highest labelling index of p53 and Ki-67.
为了研究砷诱导的皮肤癌中细胞凋亡和增殖的调控机制，我们采用免疫组织化学染色法检测了bcl-2、p53和Ki-67的表达情况。研究对象包括30例 Bowen病（BD）患者、10例基底细胞癌（BCC）患者、8例鳞状细胞癌（SCC）患者以及11例来自地方性砷中毒病区非阳光暴露部位的癌周正常皮肤（PLN）样本。结果显示：1）bcl-2在所有BCC中均呈均匀表达，在所有SCC中均无表达，在30例BD中有12例呈局灶性或均匀表达；2）p53在所有砷性皮肤癌中均有表达，BD的标记指数为75±14%，BCC为50±17%，SCC为61±15%，且在所有癌周正常皮肤中也有表达，标记指数为55±24%；3）Ki-67在所有皮肤癌中均有表达，BD的标记指数为58±17%，BCC为12±7%，SCC为47±21%，在11例PLN中有9例表达，标记指数为41±24%。BCC或BD中bcl-2的表达与生发基底细胞的表型有关。BD早期发育异常细胞中bcl-2的持续表达以及癌周正常皮肤基底细胞中p53的最早表达表明，砷诱导致癌的初始步骤源于基底生发细胞。在任何类型的砷性皮肤癌中，bcl-2、p53或Ki-67的表达之间均无相互关系，但在癌周正常皮肤中p53和Ki-67的表达呈正相关。BD的p53和Ki-67标记指数最高。
