Zvegintseva E G, Malofeeva L I
Arkh Anat Gistol Embriol. 1975 Dec;69(12):57-61.
A method for calculation of the square surface area of the cerebral cortex is proposed which represents a combination of the method of averaged reconstruction after a continuous series of histological sections and the stereological method of determination of the length of any curve disposed on the surface. A comparative analysis of the proposed method with curvometrical and stereological (after Hennig's formula) method used earlier for this purpose is made taking as an example the calculation of the neocortex square surface area of some mammals. The results of calculation of the surface by the proposed method were found to coincide with curvometrical data within the range of 5% while the data obtained by stereological method of determination of the absolute square surface area differ from curvometrical data by more than 22%. The proposed method is very convenient and allows considerable acceleration in obtaining results.
本文提出了一种计算大脑皮质表面积的方法,该方法是连续系列组织学切片后平均重建法与确定表面任意曲线长度的体视学方法的结合。以某些哺乳动物新皮质表面积的计算为例,将该方法与早期用于此目的的曲线测量法和体视学方法(根据亨尼希公式)进行了对比分析。结果发现,用该方法计算的表面积结果与曲线测量数据在5%的范围内相符,而通过确定绝对表面积的体视学方法获得的数据与曲线测量数据相差超过22%。该方法非常方便,能显著加快获得结果的速度。