Nagaraja Padmarajaiah, Yathirajan Hemmige S, Sunitha Kallanchira R, Vasantha Ramanathapura A
University of Mysore. Department of Studies in Chemistry, Manasagangotri, India.
J AOAC Int. 2002 Jul-Aug;85(4):869-74.
A sensitive, rapid, and simple spectrophotometric method is described for the determination of sulfa drugs. The method is based on the formation of a red-colored product by the diazotization of sulfonamides such as sulfathiazole (SFT), sulfadiazine (SFD), sulfacetamide (SFA), sulfamethoxazole (SFMx), sulfamerazine (SFMr), sulfaguanidine (SFG), and sulfamethazine (SFMt), followed by complexation with dopamine in the presence of molybdate ions in (1 + 1) H2SO4 medium. Absorbance of the resulting red product is measured at 490-510 nm, and the product is stable for 2 days at 27 degrees C. Beer's law is obeyed in the concentration range of 0.04-8.0 microg/mL at the wavelength of maximum absorption. The method was used successfully for the determination of some sulfonamides in tablets and eye drops. Common excipients used as additives in pharmaceuticals do not interfere in the proposed method. The method offers the advantages of simplicity, rapidity, and sensitivity without the need for extraction or heating. The limits of detection and quantitation were calculated for SFT, SFD, SFA, SFMx, SFMr, SFG, and SFMt.
本文描述了一种用于测定磺胺类药物的灵敏、快速且简单的分光光度法。该方法基于磺胺类药物（如磺胺噻唑（SFT）、磺胺嘧啶（SFD）、磺胺醋酰（SFA）、磺胺甲恶唑（SFMx）、磺胺甲基嘧啶（SFMr）、磺胺脒（SFG）和磺胺二甲嘧啶（SFMt））重氮化形成红色产物，然后在（1 + 1）硫酸介质中钼酸根离子存在下与多巴胺络合。在490 - 510 nm处测量所得红色产物的吸光度，该产物在27℃下可稳定2天。在最大吸收波长处，0.04 - 8.0 μg/mL的浓度范围内符合比尔定律。该方法已成功用于测定片剂和滴眼液中的一些磺胺类药物。药物制剂中常用的辅料作为添加剂不会干扰所提出的方法。该方法具有简单、快速和灵敏的优点，无需萃取或加热。计算了SFT、SFD、SFA、SFMx、SFMr、SFG和SFMt的检测限和定量限。
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