Laboratoire Génie des Procédés et Environnement, Faculté des Sciences et Techniques de Mohammedia, Hassan II University of Casablanca, B.P. 146, 20650 Mohammedia, Morocco; ENEA, C.R. Casaccia, via Anguillarese 301, 00123 Rome, Italy.
Laboratoire Génie des Procédés et Environnement, Faculté des Sciences et Techniques de Mohammedia, Hassan II University of Casablanca, B.P. 146, 20650 Mohammedia, Morocco.
Spectrochim Acta A Mol Biomol Spectrosc. 2017 Jun 15;181:276-285. doi: 10.1016/j.saa.2017.03.061. Epub 2017 Mar 28.
The authors have developed a sensitive spectrophotometric method for determination of sulfonamide derivatives such as sulfanilamide (SAA), sulfadiazine (SDZ), sulfacetamide (SCT) sulfamethoxazole (SMX), sulfamerazine (SMR), sulfadimethoxine (SDX), sulfamethiazole (SMT) and Sulfathiazole (STZ). This method is based on the Bratton-Marshall reaction, which involves the diazotization of sulfonamides with sodium nitrite under acidic conditions, followed by coupling with N-(1-naphtyl) ethylenediamine dihydrochloride (NED) to form a pink colored compound. Therefore, the Bratton-Marshall method was modified by optimizing the reaction conditions, which allows us to determine a low concentration range of sulfonamides compared to the reported methods. The limits of detection and quantification obtained were 0.019-0.05 and 0.06-0.16μgmL, respectively. In comparison with other reported methods using different coupling agents, the proposed method was found to be the most simple and sensitive for sulfonamides determination. In this paper, the modified method was successfully employed for the determination of sulfonamides in drinking water, seawater and pharmaceutical and veterinary formulations. The purpose of this work is to optimize and develop a simple method for extraction and concentration of sulfonamides present as residues in seawater and their quantification with the recommended spectrophotometric method. Solid phase extraction (SPE) of sulfonamides from seawater samples was evaluated using Oasis HLB cartridges (3mL, 540mg). The recovery efficiency was investigated in the sulfonamides concentration range comprised between 0.19 and 126ngmL. The ease of use of this extraction method makes it very useful for routine laboratory work.
作者开发了一种灵敏的分光光度法，用于测定磺胺类衍生物，如磺胺（SAA）、磺胺嘧啶（SDZ）、磺胺醋酰（SCT）、磺胺甲恶唑（SMX）、磺胺二甲嘧啶（SMR）、磺胺间甲氧嘧啶（SDX）、磺胺噻唑（SMT）和磺胺脒（STZ）。该方法基于 Bratton-Marshall 反应，该反应涉及在酸性条件下用亚硝酸钠将磺胺类化合物重氮化，然后与 N-(1-萘基)乙二胺二盐酸盐（NED）偶联，形成粉红色化合物。因此，我们通过优化反应条件对 Bratton-Marshall 法进行了改进，与报道的方法相比，该方法能够测定磺胺类化合物的低浓度范围。获得的检测限和定量限分别为 0.019-0.05 和 0.06-0.16μgmL。与使用不同偶联剂的其他报道方法相比，该方法被发现是最简单和最灵敏的磺胺类化合物测定方法。本文采用改进后的方法成功测定了饮用水、海水和药物及兽用制剂中的磺胺类化合物。本工作旨在优化并开发一种简单的方法，用于从海水中提取和浓缩磺胺类化合物作为残留物质，并采用推荐的分光光度法对其进行定量。采用 Oasis HLB 小柱（3mL，540mg）对海水中磺胺类化合物进行固相萃取（SPE）。考察了 0.19-126ngmL 范围内磺胺类化合物浓度对回收率的影响。该萃取方法操作简便，非常适用于常规实验室工作。
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