Woollard David C, Indyk Harvey E
AgriQuality NZ Ltd., Auckland, New Zealand.
J AOAC Int. 2002 Jul-Aug;85(4):945-51.
A simplified, simultaneous determination of vitamins B1, B2, B3, and B6 in supplemented infant formulas was developed from a single deproteinized sample extract, with analysis by reversed-phase, ion-pair chromatography with an acidified methanol-water mobile phase. The dioctylsulfosuccinate counter-ion facilitates unique retention of the pyridine-based vitamins (niacinamide and pyridoxine) and allows for concurrent measurement of both the pyridoxal and riboflavin 5'-phosphate endogenous components of milk. Other naturally occurring undetected vitamin congeners have minimal analytical significance. UV detection is used for niacinamide, and programmed fluorescence detection is used for riboflavin and the B6 vitamins. Thiamine is routinely determined sequentially under modified elution conditions.
从单一的脱蛋白样品提取物中开发出一种简化的同时测定强化婴儿配方奶粉中维生素B1、B2、B3和B6的方法，采用反相离子对色谱法，以酸化的甲醇-水流动相进行分析。二辛基磺基琥珀酸反离子有助于基于吡啶的维生素（烟酰胺和吡哆醇）的独特保留，并允许同时测量牛奶中吡哆醛和核黄素5'-磷酸的内源性成分。其他天然存在的未检测到的维生素同系物的分析意义极小。烟酰胺采用紫外检测，核黄素和B6维生素采用程序荧光检测。硫胺素通常在改良的洗脱条件下依次测定。
