Walker M C, Carpenter B E, Cooper E L
J Pharm Sci. 1981 Jan;70(1):99-101. doi: 10.1002/jps.2600700124.
A high-pressure liquid chromatographic assay was developed for the determination of four water-soluble vitamins: niacinamide, pyridoxine, thiamine, and riboflavin. The four vitamins are assayed simultaneously in multivitamin products not containing minerals. Thiamine currently is not quantitated in formulations containing minerals because it is not stable under the extraction conditions. The method was applied to the analysis of at least 12 different multivitamin products, including various formulations of sterile products, fluids, compressed tablets, and coated, compressed tablets. The method is stability indicating and is applicable to single-tablet assays.
已开发出一种高压液相色谱分析法，用于测定四种水溶性维生素：烟酰胺、吡哆醇、硫胺素和核黄素。在不含矿物质的多种维生素产品中可同时测定这四种维生素。目前，在含有矿物质的制剂中硫胺素无法进行定量测定，因为它在提取条件下不稳定。该方法应用于至少12种不同的多种维生素产品的分析，包括无菌产品、液体、压制片和包衣压制片的各种剂型。该方法具有稳定性指示作用，适用于单片剂分析。
