McWilliam Leitch E C, Stewart C S
Rowett Research Institute, Bucksburn, Aberdeen, UK.
Lett Appl Microbiol. 2002;35(3):176-80. doi: 10.1046/j.1472-765x.2002.01164.x.
To examine the effect of temperature on the antimicrobial efficacy of lactate and propionate against O157 and non-O157 Escherichia coli isolates.
Lactate and, to a lesser extent, propionate effectively reduced viability at 37 degrees C. Ethanol enhanced this effect. Reducing the temperature to 20 or 5 degrees C caused an increase in survival in the presence of these organic acids with or without ethanol. At 20 degrees C the deltapH, membrane potential and intracellular lactate anion concentration were less than at 37 degrees C.
The efficacy of lactate and propionate against E. coli O157 and non-O157 isolates is reduced at lower temperatures, perhaps due to the reduction in the deltapH, membrane potential and intracellular lactate anion concentration.
These findings suggest that the usefulness of organic acids as decontaminants for E. coli O157 is temperature dependent.
研究温度对乳酸盐和丙酸盐针对O157及非O157大肠杆菌分离株的抗菌效果的影响。
乳酸盐以及在较小程度上丙酸盐在37℃时能有效降低细菌活力。乙醇可增强此效果。将温度降至20℃或5℃时，无论有无乙醇存在，在这些有机酸环境下细菌存活率都会增加。在20℃时，ΔpH、膜电位和细胞内乳酸根阴离子浓度均低于37℃时。
较低温度下乳酸盐和丙酸盐针对大肠杆菌O157和非O157分离株的效果降低，这可能是由于ΔpH、膜电位和细胞内乳酸根阴离子浓度降低所致。
这些发现表明有机酸作为大肠杆菌O157去污剂的效用取决于温度。
