小鼠基因组的物理图谱。

A physical map of the mouse genome.

作者信息

Gregory Simon G, Sekhon Mandeep, Schein Jacqueline, Zhao Shaying, Osoegawa Kazutoyo, Scott Carol E, Evans Richard S, Burridge Paul W, Cox Tony V, Fox Christopher A, Hutton Richard D, Mullenger Ian R, Phillips Kimbly J, Smith James, Stalker Jim, Threadgold Glen J, Birney Ewan, Wylie Kristine, Chinwalla Asif, Wallis John, Hillier LaDeana, Carter Jason, Gaige Tony, Jaeger Sara, Kremitzki Colin, Layman Dan, Maas Jason, McGrane Rebecca, Mead Kelly, Walker Rebecca, Jones Steven, Smith Michael, Asano Jennifer, Bosdet Ian, Chan Susanna, Chittaranjan Suganthi, Chiu Readman, Fjell Chris, Fuhrmann Dan, Girn Noreen, Gray Catharine, Guin Ran, Hsiao Letticia, Krzywinski Martin, Kutsche Reta, Lee Soo Sen, Mathewson Carrie, McLeavy Candice, Messervier Steve, Ness Steven, Pandoh Pawan, Prabhu Anna-Liisa, Saeedi Parvaneh, Smailus Duane, Spence Lorraine, Stott Jeff, Taylor Sheryl, Terpstra Wesley, Tsai Miranda, Vardy Jill, Wye Natasja, Yang George, Shatsman Sofiya, Ayodeji Bola, Geer Keita, Tsegaye Getahun, Shvartsbeyn Alla, Gebregeorgis Elizabeth, Krol Margaret, Russell Daniel, Overton Larry, Malek Joel A, Holmes Mike, Heaney Michael, Shetty Jyoti, Feldblyum Tamara, Nierman William C, Catanese Joseph J, Hubbard Tim, Waterston Robert H, Rogers Jane, de Jong Pieter J, Fraser Claire M, Marra Marco, McPherson John D, Bentley David R

机构信息

The Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.

出版信息

Nature. 2002 Aug 15;418(6899):743-50. doi: 10.1038/nature00957. Epub 2002 Aug 4.

DOI:10.1038/nature00957

PMID:12181558

Abstract

A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.

摘要

基因组的物理图谱是导航的重要指南，可确定染色体DNA中任何基因或其他标志物的位置。我们构建了小鼠基因组的物理图谱，它包含296个重叠细菌克隆的重叠群和16992个独特标记。基于51486个同源匹配，将小鼠重叠群与人类基因组序列进行比对，从而能够利用两个基因组保守的同线性（染色体区域间的对应关系）来加速小鼠图谱的构建。该图谱为全基因组鸟枪法序列数据的组装提供了框架，以及用于生成参考序列的克隆拼接路径。在这种分辨率水平上定义人鼠比对，能够识别与人类基因组中几乎任何位置相对应的小鼠克隆。人类序列可用于采用相同策略促进构建其他哺乳动物基因组图谱。