Oda Yasushi, Tanaka Yuji, Naruse Takayoshi, Sasanabe Ryuichiro, Tsubamoto Mari, Funahashi Hiroomi
Department of Breast and Endocrine Surgery, Aichi Medical University, Nagakute-cho, Aichi-gun, Japan.
Surg Today. 2002;32(8):690-4. doi: 10.1007/s005950200128.
Somatostatin analogs have been administered to patients with pancreatic endocrine tumors in an attempt to inhibit hormone hypersecretion and prevent tumor growth. It is speculated that their efficacy is correlated with the expression of specific subtypes of somatostatin receptors. The aim of this study was to immunohistochemically evaluate the expression of somatostatin receptor subtypes in human pancreatic endocrine tumors, and to determine whether the expression of these subtypes is correlated with the effectiveness of the somatostatin analogs.
Somatostatin receptor subtypes 1, 2, and 3 (sst 1, 2, and 3) were immunohistochemically investigated in seven pancreatic endocrine tumors: four insulinomas, one VIPoma, and two nonfunctioning tumors associated with multiple endocrine neoplasia type I, using paraffin sections. Three of the four patients with insulinoma were given an octreotide injection.
Cells were homogeneously stained in the tumor region. More than 85% of the specimens expressed sst 1, 2, and 3. There was no difference among the immunohistochemical stainings of somatostatin receptor subtypes according to most tumor characteristics; however, the expression of sst 2 was extremely positive, and the expression of sst 3 was moderately positive in the specimen from a patient in whom the octreotide injection had proven very effective.
These findings indicate that the efficacy of octreotide may be correlated with the density of sst 2 and 3 in an immunohistological study using paraffin sections.
已对胰腺内分泌肿瘤患者使用生长抑素类似物，试图抑制激素分泌过多并防止肿瘤生长。据推测，它们的疗效与生长抑素受体特定亚型的表达相关。本研究的目的是通过免疫组织化学方法评估生长抑素受体亚型在人胰腺内分泌肿瘤中的表达，并确定这些亚型的表达是否与生长抑素类似物的疗效相关。
使用石蜡切片，对7例胰腺内分泌肿瘤进行免疫组织化学研究，检测生长抑素受体亚型1、2和3（sst 1、2和3），其中包括4例胰岛素瘤、1例血管活性肠肽瘤和2例与I型多发性内分泌腺瘤相关的无功能肿瘤。4例胰岛素瘤患者中的3例接受了奥曲肽注射。
肿瘤区域细胞呈均匀染色。超过85%的标本表达sst 1、2和3。根据大多数肿瘤特征，生长抑素受体亚型的免疫组织化学染色之间没有差异；然而，在奥曲肽注射已证明非常有效的患者的标本中，sst 2的表达极强，sst 3的表达为中度阳性。
这些发现表明，在使用石蜡切片的免疫组织学研究中，奥曲肽的疗效可能与sst 2和3的密度相关。
