[外源性透明质酸对伤口愈合的影响]
[Effect of exogenous hyaluronan on wound healing].
作者信息
Liu Da-lie, Li Xi-jun, Zhang Yang, Li Yu-xia
机构信息
Department of Plastic Surgery, Shenyang Army General Hospital, Shenyang, Liaoning, P. R. China 110016.
出版信息
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Jul;16(4):256-8.
OBJECTIVE
To investigate the mechanism of impeded wound healing by exogenous hyaluronan (HA).
METHODS
Wound healing models were established on 18 adult rabbit ears, which were randomly divided into 3 groups, the 2% HA treated-group (group A), the 1% HA treated-group(group B), and the PBS control group (group C). The process of wound healing was observed morphologically and histologically. The expression of alpha-smooth muscle actin in fibroblast was measured by immunohistochemical method.
RESULTS
- The mean values of wound healing time of groups A, B and C were (11.7 +/- 0.6), (11.3 +/- 0.6), and (10.8 +/- 1.0) days respectively. Wound contraction was greater in group C than in group A and group B. 2. Compared with PBS controls, the collagen fibril was slender and arrayed regularly in HA treated wound. 3. The expression of alpha-smooth muscle actin was greater in group C than in groups A and B.
CONCLUSION
It is one of reasons of impeded wound healing that exogenous HA inhibits the expression of alpha-smooth muscle protein and wound contraction. There exists dose-dependent effect.
目的
探讨外源性透明质酸（HA）阻碍伤口愈合的机制。
方法
在18只成年兔耳上建立伤口愈合模型，随机分为3组，即2% HA治疗组（A组）、1% HA治疗组（B组）和PBS对照组（C组）。从形态学和组织学方面观察伤口愈合过程。采用免疫组织化学方法检测成纤维细胞中α-平滑肌肌动蛋白的表达。
结果
- A组、B组和C组伤口愈合时间的平均值分别为（11.7±0.6）天、（11.3±0.6）天和（10.8±1.0）天。C组伤口收缩程度大于A组和B组。2. 与PBS对照组相比，HA处理伤口处的胶原纤维更细且排列规则。3. C组α-平滑肌肌动蛋白的表达高于A组和B组。
结论
外源性HA抑制α-平滑肌蛋白的表达和伤口收缩是阻碍伤口愈合的原因之一。存在剂量依赖性效应。
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