Parker Noah P, Bailey Samual S, Walner David L
Department of Otolaryngology-Head and Neck Surgery, University of Minnesota, Minneapolis, Minnesota, USA.
Laryngoscope. 2009 Apr;119(4):734-9. doi: 10.1002/lary.20131.
To evaluate basic fibroblast growth factor-2 (BFGF) and hyaluronic acid (HA) effects on a surgically produced tracheal defect.
Animal model.
Anterior punch lesions were created on 33 male rabbits divided into three equal groups. Group 1 (Control) received a normal saline (NS)-soaked collagen sponge directly on the defect, group 2 (HA alone) a NS-soaked HA sponge, and group 3 (BFGF+HA) a 10 ng/mL BFGF-soaked HA sponge. Sponge fixation and surgical closure were followed by a 90-day healing period, then animal sacrifice. Harvested tracheas were fixed, imbedded, sectioned, and stained with hematoxylin and eosin or safranin-O. Histopathological measures included: chondrocyte death, chondrocyte proliferation (both reported as number of rabbits displaying death or proliferation per group/total rabbits per group [%]), connective tissue (CT) organization (graded on a scale), epithelial closure (1-3 scale), and inflammation (1-4 scale). The final three measures are reported as an average grade per group, where values closer to 1 signify improved healing, whereas higher values signify poorer healing.
Chondrocyte death: Control, 8/11 (72.7%); HA alone, 5/11 (45.5%); BFGF+HA 5/11 (45.5%). Chondrocyte proliferation: 3/11 (27.3%), 7/11 (63.6%), and 9/11 (81.8%), respectively. CT organization: 2.00, 1.82, 1.64. Epithelial closure: 1.18, 1.09, and 1.00. Inflammation: 2.82, 1.82, and 1.73. Statistical comparisons: significantly improved chondrocyte proliferation for BFGF+HA (P = .015) and reduced inflammation for HA alone (P = .011) and BFGF+HA (P = .004) compared to controls.
BFGF+HA applied to an anterior tracheal defect significantly improves chondrocyte proliferation; HA alone and BFGF+HA significantly reduce inflammation. BFGF+HA and HA alone may improve tracheal wound healing.
评估碱性成纤维细胞生长因子-2（BFGF）和透明质酸（HA）对手术造成的气管缺损的影响。
动物模型。
在33只雄性兔子身上制造前穿孔损伤，并将其平均分为三组。第1组（对照组）在缺损处直接放置用生理盐水（NS）浸泡的胶原海绵，第2组（单独使用HA组）放置用NS浸泡的HA海绵，第3组（BFGF+HA组）放置用10 ng/mL BFGF浸泡的HA海绵。海绵固定并手术缝合后，进行90天的愈合期，然后处死动物。取出的气管进行固定、包埋、切片，并用苏木精和伊红或番红O染色。组织病理学测量指标包括：软骨细胞死亡、软骨细胞增殖（均以每组显示死亡或增殖的兔子数量/每组兔子总数 [%] 报告）、结缔组织（CT）结构（按等级评分）、上皮闭合（1-3级评分）和炎症（1-4级评分）。最后三项指标以每组的平均等级报告，数值越接近1表示愈合越好，数值越高表示愈合越差。
软骨细胞死亡：对照组，8/11（72.7%）；单独使用HA组，5/11（45.5%）；BFGF+HA组，5/11（45.5%）。软骨细胞增殖：分别为3/11（27.3%）、7/11（63.6%）和9/11（81.8%）。CT结构：2.00、1.82、1.64。上皮闭合：1.18、1.09和1.00。炎症：2.82、1.82和1.73。统计学比较：与对照组相比，BFGF+HA组软骨细胞增殖显著改善（P = 0.015），单独使用HA组和BFGF+HA组炎症减轻（单独使用HA组P = 0.011，BFGF+HA组P = 0.004）。
应用于气管前部缺损的BFGF+HA可显著改善软骨细胞增殖；单独使用HA以及BFGF+HA均可显著减轻炎症。单独使用HA和BFGF+HA可能改善气管伤口愈合。
