Tang Zhao-hui, Hou Chun-lin, Chen Qing-quan
Department of Orthopedic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai, P. R. China 200003.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2002 Jul;16(4):259-61.
To compare the effect and coverage of bacteriostasis of chitosan and sodium hyaluronate.
Each of the five bacteria, Proteus mirabilis, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Staphylococcus aureus, was cultivated for 33 tubes of broth culture. Leaving three tubes each group as control group, ploidy diluted concentration of high relative molecular weight chitosan, low relative molecular weight chitosan and sodium hyaluronate were added respectively in the broth culture. All the tubes were cultivated for 18 hours at 37 degrees C with homeothermia. Then the growth of bacteria was observed.
The minimal inhibitory concentrations (MIC) of high relative molecular weight chitosan were: Proteus mirabilis 0.031%, Escherichia coli 0.063%, Candida albicans 0.063%, Pseudomonas aeruginosa 0.063%, Staphylococcus aureus 0.063%; and the MIC of low relative molecular weight chitosan were: Proteus mirabilis 0.125%, Escherichia coli 0.025%, Candida albicans 0.25%, Pseudomonas aeruginosa 0.25%, Staphylococcus aureus 0.125%; bacteria grew well in each tube of sodium hyaluronate group and control group.
The above results show that sodium hyaluronate has no bacteriostasis, while chitosan has bacteriostasis on broad spectrum and high relative molecular weigh: chitosan has stronger effect.
比较壳聚糖和透明质酸钠的抑菌效果及覆盖范围。
将奇异变形杆菌、大肠埃希菌、白色念珠菌、铜绿假单胞菌、金黄色葡萄球菌这五种细菌各培养33管肉汤培养物。每组留3管作为对照组，分别向肉汤培养物中加入高相对分子质量壳聚糖、低相对分子质量壳聚糖和透明质酸钠的倍比稀释浓度液。所有试管于37℃恒温培养18小时，然后观察细菌生长情况。
高相对分子质量壳聚糖的最低抑菌浓度（MIC）分别为：奇异变形杆菌0.031%、大肠埃希菌0.063%、白色念珠菌0.063%、铜绿假单胞菌0.063%、金黄色葡萄球菌0.063%；低相对分子质量壳聚糖的MIC分别为：奇异变形杆菌0.125%、大肠埃希菌0.025%、白色念珠菌0.25%、铜绿假单胞菌0.25%、金黄色葡萄球菌0.125%；透明质酸钠组和对照组的各试管中细菌生长良好。
上述结果表明，透明质酸钠无抑菌作用，而壳聚糖具有广谱抑菌作用，且高相对分子质量壳聚糖的抑菌效果更强。
