Moreno-Alvarez Mario José, Viloria Matos Alfredo, López Eliezer, Belén Douglas
Laboratorio de Biomoléculas, Ingenieria de Alimentos, Universidad Simón Rodríguez-Estado Carabobo, Venezuela.
Arch Latinoam Nutr. 2002 Jun;52(2):181-6.
In this research the chemical stability of total anthocyanins in three pasteurized juices elaborated from 12% of blackberry (Rubus glaucus Benth) pulp, and addition of ascorbic acid (Formulation A: 0.1%, Formulation B: 0.05% and Formulation C: 0.01%), was evaluated by means of absorption visible spectra (400-580 nm). Physicol-chemical characterization (acidity, soluble solids content in degree Brix, pH), and count of mesophilic microorganism, fungi, yeasts, fecal coliforms (PMN/mL) and Escherichia coli, were evaluated. Sensorial parameters (color, smell, flavor) were investigated by means of un-trained panel using a hedonic scale (Fridman, P < 0.05). The study was performed during storage for 9 days. The total anthocyanins were reported as pelargonidin-3-glycoside g/L, and no significant differences were founded among the evaluated in each formulation during storage (P > 0.05). Bactocromic effect due to oxidation as not observed. Acidity (6.0-7.2 mL NaOH 0.079 N), soluble solids content (9.0-9.8 degrees Brix) and pH (3.4) did not show significant differences (P > 0.05). The microbiological evaluation showed minimum values for pasturized products (fungi CFU/mL < 10, yeast CFU/mL < 10, fecal coliforms CFU/mL < 10 and mesophilic microorganism CFU/mL between 120-140 on first day in storage). Sensorial analysis did not show significant differences (Fridman, P > 0.05).
本研究通过可见吸收光谱(400 - 580 nm)评估了三种巴氏杀菌果汁中总花青素的化学稳定性。这三种果汁由12%的黑莓(Rubus glaucus Benth)果肉制成,并添加了抗坏血酸(配方A:0.1%,配方B:0.05%,配方C:0.01%)。对其进行了理化特性分析(酸度、°Brix度的可溶性固形物含量、pH值)以及嗜温微生物、真菌、酵母菌、粪大肠菌群(每毫升菌落形成单位)和大肠杆菌的计数。通过未经训练的评价小组使用享乐量表(弗里德曼检验,P < 0.05)对感官参数(颜色、气味、风味)进行了调查。该研究在储存9天期间进行。总花青素以天竺葵素 - 3 - 糖苷克/升表示,在储存期间各配方评估结果之间未发现显著差异(P > 0.05)。未观察到因氧化导致的变色效应。酸度(6.0 - 7.2毫升0.079 N氢氧化钠)、可溶性固形物含量(9.0 - 9.8°Brix)和pH值(3.4)未显示出显著差异(P > 0.05)。微生物学评估显示巴氏杀菌产品的各项数值最低(储存第一天真菌每毫升菌落形成单位<10,酵母菌每毫升菌落形成单位<10,粪大肠菌群每毫升菌落形成单位<10,嗜温微生物每毫升菌落形成单位在120 - 140之间)。感官分析未显示出显著差异(弗里德曼检验,P > 0.05)。