Tabuchi Tomizo, Nakao Yoshiaki, Sakihama Noriyuki, Kobayashi Toshimitsu
Department of Otorhinolaryngology, Nagasaki University School of Medicine, Japan.
Ann Otol Rhinol Laryngol. 2002 Aug;111(8):736-7. doi: 10.1177/000348940211100814.
It has recently been reported that in the facial canal, the facial nerve shows enhancement on gadolinium-enhanced magnetic resonance imaging in patients with clinically normal facial nerves. However, the mechanism of this enhancement has not yet been sufficiently clarified. The present study investigated the permeability of blood vessels in human cranial nerves that were obtained from surgically treated patients. The patients received an intravenous injection of sodium fluorescein 45 minutes before nerve resection. For histologic observation, the nerves were removed and frozen at -70 degrees C, and the sections were then cut at 4-microm thickness with a freezing microtome. The localization of the tracers was examined with a fluorescence microscope. Fluorescence was observed in the external nerve sheath and slightly in the endoneurium of these nerves, but was not observed within nerve fibers. These findings indicate that the vascular barrier in human peripheral nerves is incomplete.
最近有报道称，在面神经管内，临床上面神经正常的患者在钆增强磁共振成像中面神经会出现强化。然而，这种强化的机制尚未得到充分阐明。本研究调查了从接受手术治疗的患者身上获取的人类颅神经血管的通透性。患者在神经切除前45分钟接受了荧光素钠静脉注射。为了进行组织学观察，将神经取出并在-70℃下冷冻，然后用冷冻切片机切成4微米厚的切片。用荧光显微镜检查示踪剂的定位。在这些神经的神经外膜中观察到荧光，在神经内膜中也有轻微荧光，但在神经纤维内未观察到荧光。这些发现表明人类周围神经的血管屏障是不完整的。
