Kuklińska Danuta, Chodorowska Małgorzata
Zakład Bakteriologii PZH w Warszawie.
Med Dosw Mikrobiol. 2002;54(1):35-43.
Selected elements of simplified, bacteriological diagnosis of diphtheria were presented. The procedure of Corynebacterium strains isolation from diphtheria suspected persons and performing of toxin testing of potentially toxigenic isolates: C. diphtheriae, C. ulcerans and C. pseudotuberculosis were shortened. The role of selective tellurite media was underlined but Loeffler medium was rejected. Columbia blood agar plate was utilized for preliminary culture. Biochemical tests and toxin testing were performed from this medium. Presented diphtheria diagnosis scheme may have practical application for the laboratory work in Poland.
介绍了白喉简化细菌学诊断的部分要素。缩短了从疑似白喉患者中分离棒状杆菌菌株以及对潜在产毒分离株(白喉棒状杆菌、溃疡棒状杆菌和假结核棒状杆菌)进行毒素检测的程序。强调了选择性亚碲酸盐培养基的作用,但摒弃了吕氏培养基。使用哥伦比亚血琼脂平板进行初步培养。从该培养基进行生化试验和毒素检测。所提出的白喉诊断方案可能在波兰的实验室工作中有实际应用价值。