Pimenta F P, Souza M C, Pereira G A, Hirata R, Camello T C F, Mattos-Guaraldi A L
Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, RJ, Brazil.
Lett Appl Microbiol. 2008 Mar;46(3):307-11. doi: 10.1111/j.1472-765X.2007.02310.x.
To examine the value of the DNase test as an alternative procedure for differentiating Corynebacterium diphtheriae from Corynebacterium-like colonies.
DNase test medium was inoculated by spotting a loopful of bacterial growth and incubated aerobically at 37 degrees C. The DNase production was detectable following both 24 and 48 h incubation periods. The DNase activity was detected in all 91 C. diphtheriae (37 toxigenic and 54 nontoxigenic) strains examined, previously identified by both conventional biochemical methods and API Coryne System. Conversely, DNase test results were negative in 93.9% of the 564 nondiphtherial Gram-positive rod clinical strains.
The DNase test emerged as an easily interpretable and cost-effective alternative screening procedure for C. diphtheriae laboratory identification.
The method should facilitate routine laboratory diagnosis of toxigenic and nontoxigenic C. diphtheriae.
检测脱氧核糖核酸酶（DNase）试验作为区分白喉棒状杆菌与类白喉棒状杆菌菌落的替代方法的价值。
通过接种一环细菌生长物来接种DNase试验培养基，并在37℃有氧条件下孵育。在孵育24小时和48小时后均能检测到DNase的产生。在所检测的91株白喉棒状杆菌（37株产毒素菌株和54株不产毒素菌株）中均检测到了DNase活性，这些菌株先前已通过传统生化方法和API棒状杆菌系统鉴定。相反，在564株非白喉革兰氏阳性杆菌临床菌株中，93.9%的菌株DNase试验结果为阴性。
DNase试验成为一种易于解释且具有成本效益的白喉棒状杆菌实验室鉴定替代筛查方法。
该方法应有助于产毒素和不产毒素白喉棒状杆菌的常规实验室诊断。
