在新生大鼠培养星形胶质细胞中使用笼锁钙离子载体通过光解闪光诱导细胞间钙波。

Photolytic flash-induced intercellular calcium waves using caged calcium ionophore in cultured astrocytes from newborn rats.

作者信息

Iwabuchi Sadahiro, Kawahara Koichi, Makisaka Koji, Sato Hideomi

机构信息

Laboratory of Biomedical Control, Research Institute for Electronic Science, Hokkaido University, Sapporo 060-0812, Japan.

出版信息

Exp Brain Res. 2002 Sep;146(1):103-16. doi: 10.1007/s00221-002-1149-y. Epub 2002 Jul 12.

DOI:10.1007/s00221-002-1149-y

PMID:12192584

Abstract

Waves of elevated intracellular free calcium that propagate between neighboring astrocytes are important for the intercellular communication between astrocytes as well as between neurons and astrocytes. However, the mechanisms responsible for the initiation and propagation of astrocytic calcium waves remain unclear. In this study, intercellular calcium waves were evoked by focal photolysis of a caged calcium ionophore (DMNPE-caged Br A23187) in cultured astrocytes from newborn rats. The focal photolysis of the caged compound resulted in the increase in intracellular calcium in a single astrocyte, and this increase then propagated to neighboring astrocytes. We also analyzed the spatiotemporal characteristics of the intercellular calcium waves, and estimated the propagation pathways for them. The method using a caged calcium ionophore described in this study provides a new in vitro model for the analysis of intercellular calcium waves.

摘要

在相邻星形胶质细胞之间传播的细胞内游离钙升高波，对于星形胶质细胞之间以及神经元与星形胶质细胞之间的细胞间通讯非常重要。然而，负责星形胶质细胞钙波起始和传播的机制仍不清楚。在本研究中，通过对新生大鼠培养的星形胶质细胞中笼化钙离子载体（DMNPE-笼化Br A23187）进行局部光解来诱发细胞间钙波。笼化化合物的局部光解导致单个星形胶质细胞内钙增加，然后这种增加传播到相邻的星形胶质细胞。我们还分析了细胞间钙波的时空特征，并估计了它们的传播途径。本研究中描述的使用笼化钙离子载体的方法为分析细胞间钙波提供了一种新的体外模型。