Goodlett David R, Yi Eugene C
The Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103-8904, USA.
Funct Integr Genomics. 2002 Sep;2(4-5):138-53. doi: 10.1007/s10142-001-0041-3. Epub 2002 Jan 22.
Proteomics can be thought of as an attempt to understand the information encoded in genomic sequences from the perspective of proteins; i.e. the structure, function and regulation of biological processes at the protein level. In practice it stands in stark contrast to the hypothesis-driven serial approach practiced in the last century that was so successful for protein chemists and is built on the basic understanding of protein physicochemical properties developed during that era. Proteomics attempts to study biological processes comprehensively or globally by systematic parallel analysis of proteins expressed in a cell. While there are many analytical techniques in use and under development in proteomics, mass spectrometry is currently one of the field's most important discovery-based tools. This article will review some of the current approaches for qualitative and quantitative uses of tandem mass spectrometry in the field of proteomics specifically avoiding a discussion of the use of gel electrophoresis prior to mass spectrometry.
蛋白质组学可以被视为从蛋白质的角度理解基因组序列中编码信息的一种尝试；即蛋白质水平上生物过程的结构、功能和调控。实际上，它与上个世纪实践的假设驱动的串行方法形成鲜明对比，这种方法对蛋白质化学家来说非常成功，并且建立在对那个时代发展起来的蛋白质物理化学性质的基本理解之上。蛋白质组学试图通过对细胞中表达的蛋白质进行系统的平行分析来全面或整体地研究生物过程。虽然蛋白质组学中有许多正在使用和开发的分析技术，但质谱目前是该领域最重要的基于发现的工具之一。本文将综述蛋白质组学领域中串联质谱定性和定量应用的一些当前方法，特别避免讨论质谱分析之前凝胶电泳的使用。
