VerBerkmoes Nathan C, Connelly Heather M, Pan Chongle, Hettich Robert L
Genome Science & Technology Graduate School, University of Tennessee, Knoxville, TN 37996, USA.
Expert Rev Proteomics. 2004 Dec;1(4):433-47. doi: 10.1586/14789450.1.4.433.
The emergence of advanced liquid chromatography mass spectrometry technologies for characterizing very complex mixtures of proteins has greatly propelled the field of proteomics, the goal of which is the simultaneous examination of all the proteins expressed by an organism. This research area represents a paradigm shift in molecular biology by attempting to provide a top-down qualitative and quantitative view of all the proteins (including their modifications and interactions) that are essential for an organism's life cycle, rather than targeting a particular protein family. This level of global protein information about an organism such as a bacterium can be combined with genomic and metabolomic data to enable a systems biology approach for understanding how these organisms live and function.
用于表征非常复杂的蛋白质混合物的先进液相色谱质谱技术的出现，极大地推动了蛋白质组学领域的发展。蛋白质组学的目标是同时检测生物体表达的所有蛋白质。该研究领域代表了分子生物学的一种范式转变，它试图提供一种自上而下的定性和定量视角，来观察生物体生命周期中所有必需的蛋白质（包括它们的修饰和相互作用），而不是针对特定的蛋白质家族。关于细菌等生物体的这种全球蛋白质信息水平，可以与基因组和代谢组数据相结合，以实现一种系统生物学方法，用于理解这些生物体如何生存和发挥功能。
