Dierkes Paul Wilhelm, Coulon Philippe, Neumann Susanne, Schlue Wolf-Rüdiger
Institut für Neurobiologie, Heinrich-Heine-Universität Düsseldorf, Universitätsstr. 1, 40225 Düsseldorf, Germany.
Anal Bioanal Chem. 2002 Apr;373(8):762-6. doi: 10.1007/s00216-002-1415-7. Epub 2002 Jul 23.
Because changes in cell volume might disturb the normal function of animal cells, most cells are endowed with volume-regulating mechanisms. Experimentally induced changes in cell volume are often paralleled by changes in the membrane potential, which might affect a variety of transport processes across the cell membrane and, in turn, volume regulation [1, 2]. We have shown previously that multi-barrelled ion-selective microelectrodes are useful tools for measurement of cell volume and the intracellular concentrations of ions that might be relevant to volume regulation [1]. To investigate whether voltage-dependent transport processes are involved in cell-volume regulation we combined the potentiometric technique of ion-selective microelectrodes with the voltage-clamp technique. This combination enables simultaneous recording of cell volume, relevant intracellular ion concentrations, and ion currents across the cell membrane at a fixed membrane potential.
由于细胞体积的变化可能会干扰动物细胞的正常功能，大多数细胞都具备体积调节机制。实验诱导的细胞体积变化通常伴随着膜电位的变化，这可能会影响多种跨细胞膜的转运过程，进而影响体积调节[1,2]。我们之前已经表明，多管离子选择性微电极是测量细胞体积以及可能与体积调节相关的细胞内离子浓度的有用工具[1]。为了研究电压依赖性转运过程是否参与细胞体积调节，我们将离子选择性微电极的电位测量技术与电压钳技术相结合。这种结合能够在固定膜电位下同时记录细胞体积、相关的细胞内离子浓度以及跨细胞膜的离子电流。
