钾光致电离在DNA组成原子内部及附近所引发的生物学效应。

Biological effects induced by K photo-ionisation in and near constituent atoms of DNA.

作者信息

Touati A, Hervé du Penhoat M A, Fayard B, Champion C, Abel F, Gobert F, Lamoureux M, Politis M F, Martins L, Ricoul M, Sabatier L, Sage E, Chetioui A

机构信息

Universites Paris 6/7, CNRS UMR 75-88, CEA LRC No. 6, Paris, France.

出版信息

Radiat Prot Dosimetry. 2002;99(1-4):83-4. doi: 10.1093/oxfordjournals.rpd.a006845.

DOI:10.1093/oxfordjournals.rpd.a006845

PMID:12194367

Abstract

In order to assess the lethal efficiency and other biological effects of inner shell ionisations of constituent atoms of DNA ('K' events), experiments were developed at the LURE synchrotron facility using ultrasoft X rays as a probe of K events. The lethal efficiency of ultrasoft X rays above the carbon K threshold was especially investigated using V79 cells and compared with their efficiency to induce double strand breaks in dry plasmid-DNA. A correlation between the K event efficiencies for these processes is shown. Beams at 340 eV were found to be twice as efficient at killing cells than were beams at 250 eV. In addition, a rough two-fold increase of the relative biological effectiveness for dicentric + ring induction has also been observed between 250 and 340 eV radiations.

摘要

为了评估DNA组成原子内壳层电离（“K”事件）的致死效率和其他生物学效应，在卢尔同步加速器设施开展了实验，使用极软X射线作为K事件的探测手段。特别使用V79细胞研究了高于碳K阈值的极软X射线的致死效率，并将其与诱导干燥质粒DNA双链断裂的效率进行了比较。结果表明了这些过程的K事件效率之间存在相关性。发现340 eV的光束杀死细胞的效率是250 eV光束的两倍。此外，在250至340 eV辐射之间，还观察到双着丝粒+环诱导的相对生物学效应大致增加了两倍。