Hoang Linh, Bedard Sabrina, Krishna Mallela M G, Lin Yan, Englander S Walter
The Johnson Research Foundation, University of Pennsylvania School of Medicine, Philadelphia, PA 19104-6059, USA.
Proc Natl Acad Sci U S A. 2002 Sep 17;99(19):12173-8. doi: 10.1073/pnas.152439199. Epub 2002 Aug 26.
Native-state hydrogen exchange experiments under EX1 conditions can distinguish partially unfolded intermediates by their formation rates and identify the amide hydrogens exposed and protected in each. Results obtained define a cytochrome c intermediate seen only poorly before and place it early on the major unfolding pathway. Four distinct unfolding steps are found to be kinetically ordered in the same pathway sequence inferred before.
在EX1条件下进行的天然态氢交换实验能够通过部分展开中间体的形成速率来区分它们,并识别出每个中间体中暴露和受到保护的酰胺氢。所获得的结果明确了一种之前仅能模糊观察到的细胞色素c中间体,并将其置于主要展开途径的早期阶段。研究发现,在之前推断的相同途径序列中,四个不同的展开步骤在动力学上是有序的。