能量营养素对大鼠大脑皮层氨基酸代谢影响的个体发生学研究。

Ontogenetic study of the effects of energetic nutrients on amino acid metabolism of rat cerebral cortex.

作者信息

de Oliveira Karla R, Rotta Liane N, Valle Sandra C, Pilger Diogo A, Nogueira Cristina W, Feoli Ana M, Bernard Elena A, Souza Diogo O, Perry Marcos L S

机构信息

Departamento de Ciências da Saúde, UNIJUI/IJUI, RS, Brazil.

出版信息

Neurochem Res. 2002 Jun;27(6):513-8. doi: 10.1023/a:1019804804592.

DOI:10.1023/a:1019804804592

PMID:12199157

Abstract

We studied the effect of various energetic nutrients on metabolism of L-[U-14C]leucine and [1-14C]glycine in cerebral cortex of rats at different ages. At gestational age, glucose and lactate stimulated protein synthesis from L-[U-14C]leucine and [1-14C]glycine and from L-[U-14C]leucine, respectively; glucose, beta-OH-butyrate and lactate stimulated lipid synthesis from L-[U-14C]leucine. At 10 days of age, glucose, mannose, and fructose stimulated protein synthesis, and glucose and mannose stimulated oxidation to CO2 as well as lipid synthesis from L-[U-14C]leucine. In adult rats, glucose, mannose, and fructose stimulated protein synthesis from L-[U-14C]leucine and [1-14C]glycine; glutamine also markedly decreased the oxidation of L-[U-14C]leucine and [1-14C]glycine in 10-day-old and adult rats.

摘要

我们研究了不同年龄段大鼠大脑皮层中各种高能营养素对L-[U-¹⁴C]亮氨酸和[1-¹⁴C]甘氨酸代谢的影响。在胎龄时，葡萄糖和乳酸分别刺激L-[U-¹⁴C]亮氨酸和[1-¹⁴C]甘氨酸的蛋白质合成以及L-[U-¹⁴C]亮氨酸的脂质合成；葡萄糖、β-羟基丁酸和乳酸刺激L-[U-¹⁴C]亮氨酸的脂质合成。在10日龄时，葡萄糖、甘露糖和果糖刺激蛋白质合成，葡萄糖和甘露糖刺激L-[U-¹⁴C]亮氨酸氧化为二氧化碳以及脂质合成。在成年大鼠中，葡萄糖、甘露糖和果糖刺激L-[U-¹⁴C]亮氨酸和[1-¹⁴C]甘氨酸的蛋白质合成；谷氨酰胺也显著降低10日龄和成年大鼠中L-[U-¹⁴C]亮氨酸和[1-¹⁴C]甘氨酸的氧化。