使用β-环糊精流动相通过间接旋光检测法液相色谱测定食品和饮料中的醇类
Liquid chromatographic determination of alcohols in food and beverages with indirect polarimetric detection using a beta-cyclodextrin mobile phase.
作者信息
Munaf Edison, Zein Rahmiana, Jin Ji-Ye, Takeuchi Toyohide
机构信息
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Padang 25163, Indonesia.
出版信息
Anal Sci. 2002 Aug;18(8):903-6. doi: 10.2116/analsci.18.903.
An indirect polarimetric detection method for the determination of alcohols has been proposed in liquid chromatography (LC). Optically active mobile-phase additives, such as beta-cyclodextrin (beta-CD), could be used to visualize optically inactive alcohols in reversed-phase LC. The visualization of alcohols is based on a perturbation of the partition of beta-CD caused by the alcohols. The detection limits of the present system at a signal-to-noise ratio of 3 were 0.031, 0.019, 0.018, 0.013, 0.011, 0.008 and 0.008% (v/v) for ethanol, 2-propanol, 1-propanol, 2-methyl-2-propanol, 2-butanol, 2-methyl-1-propanol and 1-butanol, respectively. The method was successfully applied to the determination of ethanol present in food and beverage samples.
液相色谱（LC）中已提出一种用于测定醇类的间接偏振检测方法。光学活性流动相添加剂，如β-环糊精（β-CD），可用于在反相LC中使光学惰性的醇类可视化。醇类的可视化基于醇类对β-CD分配的扰动。在信噪比为3时，本系统对乙醇、2-丙醇、1-丙醇、2-甲基-2-丙醇、2-丁醇、2-甲基-1-丙醇和1-丁醇的检测限分别为0.031%（v/v）、0.019%（v/v）、0.018%（v/v）、0.013%（v/v）、0.011%（v/v）、0.008%（v/v）和0.008%（v/v）。该方法已成功应用于食品和饮料样品中乙醇的测定。
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