Kubota Y, Fukuda M, Ohtsuji K, Koizumi K
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Anal Biochem. 1992 Feb 14;201(1):99-102. doi: 10.1016/0003-2697(92)90179-b.
High-performance liquid chromatography with pulsed amperometric detection was applied to the determination of beta-cyclodextrin (beta-CD) and glucosyl (G)-beta-CD in human plasma. They were well resolved from each other and from background components of plasma on a polymer-based reversed-phase column with 0.6% acetonitrile aqueous solution containing 1 mM sodium hydroxide as an eluent. The samples in the effluent were detected with a pulsed amperometric detector after postcolumn alkalization. The detection limits of beta-CD and G-beta-CD in plasma at a signal-to-noise ratio of 3 were 11 and 5 pmol, respectively.
