Su Yu-Hong, Xiong Yuan-Zhu, Zhang Qin, Jiang Si-Wen, Lei Ming-Gang, Yu Li, Zheng Rong, Deng Chang-Yan
College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Yi Chuan Xue Bao. 2002;29(8):681-4.
One of the major determining factors in the price of market hogs today is backfat depth. Therefore, identification of regions of the genome affecting this trait is necessary. Gene-mapping technologies have provided scientists the necessary reagents to conduct genomewide searches for genes affecting any phenotype determined in part by the genetic makeup of the animal. Over the past few years, several experimental crosses have been used to detect quantitative triat loci (QTL) for fatness and meat quality traits in pigs. In an intercross between Large White and Meishan pigs, 81 F2 progenies in 1998 were slaughtered and phenotyped for fat deposite traits. F2 animals, their parents, and their grandparents were typed for molecular markers covering chromosomes (SSC) 1,2,3,4,6 and 7, since previous studies had revealed QTL affecting fatness traits on these chromosomes. Linear model and least square analyses were used for mapping these traits. Furthermore, a QTL accounting for imprinting effects was used. A total of 14 QTL at chromosome-wide level were detected on SSC1, 3-4, 6 and 7 for 12 fatness traits. There were evidences for imprinting effects on SSC7 for four traits at 60 cM and nearby. There were QTL for average backfat thickness (BFT) on SSC1, 4 and 7. Among them, BFT QTL on SSC1 and 7 may be the common locus for Sus scrofa. QTL for internal fat percentage was on SSC7 and QTL for average sidefat on SSC6.
目前,影响市场生猪价格的主要决定因素之一是背膘厚度。因此,有必要识别影响该性状的基因组区域。基因定位技术为科学家们提供了必要的试剂,以便在全基因组范围内搜索影响任何部分由动物基因组成决定的表型的基因。在过去几年中,已经进行了几次实验杂交,以检测猪的脂肪和肉质性状的数量性状位点(QTL)。在大白猪和梅山猪的杂交中,1998年对81头F2后代进行了屠宰,并对脂肪沉积性状进行了表型分析。由于先前的研究已经揭示了影响这些染色体上脂肪性状的QTL,因此对F2动物及其父母和祖父母进行了覆盖1、2、3、4、6和7号染色体(SSC)的分子标记分型。使用线性模型和最小二乘法分析来定位这些性状。此外,还使用了一个考虑印记效应的QTL。在SSC1、3-4、6和7号染色体上,共检测到14个全染色体水平的QTL,涉及12个脂肪性状。有证据表明,在60 cM及附近的4个性状上,SSC7存在印记效应。SSC1、4和7号染色体上存在平均背膘厚度(BFT)的QTL。其中,SSC1和7号染色体上的BFT QTL可能是野猪的共同位点。SSC7上存在内脂百分比的QTL,SSC6上存在平均边膘的QTL。