Chang B V, Shiung L C, Yuan S Y
Department of Microbiology, Soochow University, Shih Lin, Taipei 111, Taiwan, ROC.
Chemosphere. 2002 Aug;48(7):717-24. doi: 10.1016/s0045-6535(02)00151-0.
Known concentrations of phenanthrene, pyrene, anthracene, fluorene and acenapthene were added to soil samples to investigate the anaerobic degradation potential of polycyclic aromatic hydrocarbon (PAH). Consortia-treated river sediments taken from known sites of long-term pollution were added as inoculum. Mixtures of soil, consortia, and PAH (individually or combined) were amended with nutrients and batch incubated. High-to-low degradation rates for both soil types were phenanthrene > pyrene > anthracene > fluorene > acenaphthene. Degradation rates were faster in Taida soil than in Guishan soil. Faster individual PAH degradation rates were also observed in cultures containing a mixture of PAH substrates compared to the presence of a single substrate. Optimal incubation conditions were noted as pH 8.0 and 30 degrees C. Degradation was enhanced for PAH by the addition of acetate, lactate, or pyruvate. The addition of municipal sewage or oil refinery sludge to the soil samples stimulated PAH degradation. Biodegradation was also measured under three anaerobic conditions; results show the high-to-low order of biodegradation rates to be sulfate-reducing conditions > methanogenic conditions > nitrate-reducing conditions. The results show that sulfate-reducing bacteria, methanogen, and eubacteria are involved in the PAH degradation; sulfate-reducing bacteria constitute a major component of the PAH-adapted consortia.
将已知浓度的菲、芘、蒽、芴和苊添加到土壤样品中，以研究多环芳烃（PAH）的厌氧降解潜力。添加从长期污染已知地点采集的经菌群处理的河流沉积物作为接种物。用养分对土壤、菌群和PAH（单独或混合）的混合物进行改良，并进行批次培养。两种土壤类型从高到低的降解速率为菲>芘>蒽>芴>苊。泰达土壤中的降解速率比桂山土壤中的快。与单一底物存在相比，在含有PAH底物混合物的培养物中也观察到更快的单个PAH降解速率。最佳培养条件为pH 8.0和30℃。添加乙酸盐、乳酸盐或丙酮酸盐可增强PAH的降解。向土壤样品中添加城市污水或炼油厂污泥可刺激PAH降解。还在三种厌氧条件下测量了生物降解；结果表明生物降解速率从高到低的顺序为硫酸盐还原条件>产甲烷条件>硝酸盐还原条件。结果表明，硫酸盐还原菌、产甲烷菌和真细菌参与了PAH降解；硫酸盐还原菌是适应PAH的菌群的主要组成部分。
