Villanueva Laura, Haveman Shelley A, Summers Zara M, Lovley Derek R
Harvard FAS Center for Systems Biology, Harvard University, Cambridge, MA 02138, USA.
Appl Environ Microbiol. 2008 Sep;74(18):5850-3. doi: 10.1128/AEM.00399-08. Epub 2008 Jul 25.
Previous studies have suggested that levels of transcripts for dsrA, a gene encoding a subunit of the dissimilatory sulfite reductase, are not directly related to the rates of sulfate reduction in sediments under all conditions. This phenomenon was further investigated with chemostat-grown Desulfovibrio vulgaris. Under sulfate-limiting conditions, dsrA transcript levels increased as the bulk rates of sulfate reduction in the chemostat increased, but transcript levels were similar at all sulfate reduction rates under electron donor-limiting conditions. When both electron donor- and electron acceptor-limiting conditions were considered, there was a direct correspondence between dsrA transcript levels and the rates of sulfate reduction per cell. These results suggest that dsrA transcript levels may provide important information on the metabolic state of sulfate reducers.
先前的研究表明，编码异化亚硫酸盐还原酶一个亚基的基因dsrA的转录本水平，在所有条件下都与沉积物中硫酸盐还原速率没有直接关系。利用恒化器培养的普通脱硫弧菌对这一现象进行了进一步研究。在硫酸盐限制条件下，随着恒化器中硫酸盐还原总体速率的增加，dsrA转录本水平升高，但在电子供体限制条件下，所有硫酸盐还原速率下的转录本水平相似。当同时考虑电子供体和电子受体限制条件时，dsrA转录本水平与每个细胞的硫酸盐还原速率之间存在直接对应关系。这些结果表明，dsrA转录本水平可能提供有关硫酸盐还原菌代谢状态的重要信息。
