未受精人类卵母细胞和极体的染色体荧光原位杂交分析

Chromosomal FISH analysis of unfertilized human oocytes and polar bodies.

作者信息

Honda Nao, Miharu Norio, Hara Tetsuaki, Samura Osamu, Honda Hiroshi, Ohama Koso

机构信息

Department of Obstetrics and Gynecology, Hiroshima University School of Medicine, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan.

出版信息

J Hum Genet. 2002;47(9):488-91. doi: 10.1007/s100380200071.

DOI:10.1007/s100380200071

PMID:12202989

Abstract

The incidence of chromosomal aneuploidy was studied in 208 unfertilized metaphase II human oocytes from an in vitro fertilization program by fluorescence in situ hybridization using probes for chromosomes 18, 21, and X. Chromosome spreads were prepared by a gradual fixation-air-drying method. We obtained analyzable results from 183 oocytes and 93 polar bodies; 167 oocytes (91%) were normal, 11 (6%) were diploid, and 5 (3%) were aneuploid. Of the five aneuploid oocytes, four involved chromosome 21, and one involved the X chromosome. In this study, oocyte aneuploidy caused by both nondisjunction of bivalent chromosomes and predivision of univalent chromosomes was observed. The aneuploidy rate (9.8%) in the oocytes from women aged >==35 years was significantly higher than that (0.7%) in those aged 23 to 34 years ( P = 0.0017).

摘要

通过使用针对18号、21号和X染色体的探针进行荧光原位杂交，对来自体外受精项目的208个未受精的中期II期人类卵母细胞的染色体非整倍体发生率进行了研究。采用逐步固定-空气干燥法制备染色体涂片。我们从183个卵母细胞和93个极体中获得了可分析的结果；167个卵母细胞（91%）正常，11个（6%）为二倍体，5个（3%）为非整倍体。在这5个非整倍体卵母细胞中，4个涉及21号染色体，1个涉及X染色体。在本研究中，观察到了由二价染色体不分离和单价染色体提前分离导致的卵母细胞非整倍体。年龄≥35岁女性的卵母细胞中非整倍体率（9.8%）显著高于23至34岁女性的卵母细胞（0.7%）（P = 0.0017）。