Chen Wen, Mettler Liselotte
Department of Gynecology and Obstetrics, Tongji Hospital of Tongji Medical College, Huazhong Science and Technology University, Wuhan, Hubei, 430030 PR China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2004 Jun;21(3):203-6.
To investigate the mechanism of oocyte aneuploidy formation.
The unfertilized oocytes were fixed 1-3 days after oocyte retrieval. Multicolor fluorescence in situ hybridization (M-FISH) was performed according to the Vysis protocol to check the chromosome status in oocytes by using centromeric enumerator probes for chromosomes 16, 18 and locus-specific probes for chromosomes 13, 21 and 22.
47% oocytes were found to be normal, while 53 % oocytes were abnormal. Nondisjunction was found in 22(18%) oocytes, unbalanced predivision of chromatids in 15(12%) oocytes, balanced predivision of chromatids in 45(36%) oocytes. The balanced predivision rate in oocytes aged in vitro>24h was much higher than that in oocytes aged in vitro<or=24h (P<0.01).
Both nondisjunction, balanced and unbalanced predivision of chromatids are involved in the oocyte aneuploidy formation. Balanced predivision is related to the time in culture.
研究卵母细胞非整倍体形成的机制。
在取卵后1 - 3天固定未受精的卵母细胞。根据Vysis方案进行多色荧光原位杂交（M - FISH），通过使用16号、18号染色体的着丝粒计数探针以及13号、21号和22号染色体的位点特异性探针来检查卵母细胞中的染色体状态。
发现47%的卵母细胞正常，而53%的卵母细胞异常。在22个（18%）卵母细胞中发现了染色体不分离，15个（12%）卵母细胞中存在染色单体不平衡前期分裂，45个（36%）卵母细胞中存在染色单体平衡前期分裂。体外培养时间>24小时的卵母细胞中染色单体平衡前期分裂率远高于体外培养时间≤24小时的卵母细胞（P<0.01）。
染色体不分离、染色单体平衡和不平衡前期分裂均参与了卵母细胞非整倍体的形成。平衡前期分裂与培养时间有关。
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