Ito Yukishige, Manabe Shino
RIKEN, The Institute of Physical and Chemical Research, Wako-shi, Saitama, Japan.
Chemistry. 2002 Jul 15;8(14):3076-84. doi: 10.1002/1521-3765(20020715)8:14<3076::AID-CHEM3076>3.0.CO;2-N.
Low molecular weight poly(ethylene glycol) (LWPEG) has been found to be useful as a "tag" in oligosaccharide synthesis due to its high polarity. Real-time reaction monitoring was achieved by use of MALDI-TOF MS in the glycosylation reactions and color tests in the deprotection of the chloroacetyl group. Further, a cysteine-supported insoluble resin enables the purification of the chloroacetyl-bound compounds on soluble PEG.
低分子量聚乙二醇（LWPEG）因其高极性而被发现可作为寡糖合成中的“标签”。在糖基化反应中使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）以及在氯乙酰基脱保护过程中进行颜色测试实现了实时反应监测。此外，一种半胱氨酸负载的不溶性树脂能够在可溶性聚乙二醇上纯化氯乙酰基结合的化合物。
