Blanco J, Acosta C P, Bermúdez de la Puente M, Salgado C
Centro de Investigacións Mariñas, Pedras de Corón s/n, Aptdo. 13, 36620, Vilanova de Arousa, Spain.
Aquat Toxicol. 2002 Oct 2;60(1-2):111-21. doi: 10.1016/s0166-445x(01)00274-0.
The depuration kinetics of the domoic acid of four body fractions (digestive gland, adductor muscle, gonad+kidney and gills+mantle) of the scallop Pecten maximus was studied over 295 days. The scallops, which had acquired the toxins during a Pseudo-nitzschia australis episode that took place the week before the beginning of the experiment, were maintained in tanks with running seawater. All the body fractions, except the adductor muscle, decreased their domoic acid burden throughout the experiment. The amount of toxin in the muscle dropped sharply at the start of the experiment but increased again at the end, to levels that were higher than the initial ones. Several dynamic models of depuration kinetics, which included the depuration of each fraction (excluding the adductor muscle) and the transfers between them, were constructed, implemented and fitted to the data to obtain their parameters. The estimated depuration rates were very low, both considering and not considering the transfer of toxin between organs or the effect of weight loss. There were strong differences in the domoic acid burden of the body fractions studied but not between their depuration rates. No net transfer from the digestive gland, the tissue with highest domoic acid concentration, to the other fractions was found, as the inclusion of these processes in the models produced only a marginally better fit to the data. The depuration of domoic acid was slightly, but significantly, affected by biomass. Weight loss induced domoic acid loss, suggesting that part of the depuration may be produced by the direct loss of bivalve cells. The concentration or dilution effect, due to decreases or increases in biomass, documented for other species and toxins, has little importance in Pecten maximus.
在295天的时间里，研究了大扇贝（Pecten maximus）四个身体部分（消化腺、闭壳肌、性腺 + 肾脏以及鳃 + 外套膜）中软骨藻酸的净化动力学。这些扇贝在实验开始前一周发生的一次澳大利亚拟菱形藻事件中摄取了毒素，之后被置于装有流动海水的水槽中饲养。在整个实验过程中，除闭壳肌外，所有身体部分的软骨藻酸含量均有所下降。肌肉中的毒素含量在实验开始时急剧下降，但在实验结束时又有所增加，且高于初始水平。构建、实施了几个净化动力学的动态模型，这些模型包括每个部分（不包括闭壳肌）的净化以及它们之间的转移，并将其与数据进行拟合以获得参数。无论是否考虑器官间毒素的转移或体重减轻的影响，估计的净化速率都非常低。所研究的身体部分的软骨藻酸含量存在显著差异，但它们的净化速率并无差异。未发现从软骨藻酸浓度最高的组织——消化腺向其他部分的净转移，因为在模型中纳入这些过程仅使数据拟合略有改善。软骨藻酸的净化受到生物量的轻微但显著的影响。体重减轻导致软骨藻酸损失，这表明部分净化可能是由于双壳贝类细胞的直接损失所致。对于其他物种和毒素所记录的因生物量减少或增加而产生的浓度或稀释效应，在大扇贝中并不重要。
