Anwer A, El-Aaser A A, Hassanein S, Hassanein M
Acta Biol Acad Sci Hung. 1975;26(1-2):105-10.
A lead method for demonstrating alkaline phosphatase is described. The method is based on direct precipitation of lead as lead phosphatase at pH 9.5, the pH optimum of the enzyme. Stable incubation medium was achieved by using tartrate, instead of maleate, as chelating for lead. The method was found to be suitable for visualization of alkaline phosphatase in different types of tissues.
本文描述了一种用于显示碱性磷酸酶的铅法。该方法基于在pH 9.5（该酶的最适pH值）下将铅直接沉淀为磷酸铅酶。通过使用酒石酸盐而非马来酸盐作为铅的螯合剂，获得了稳定的孵育介质。结果发现该方法适用于观察不同类型组织中的碱性磷酸酶。
