el-Aaser A A, Hindawy D S, Hammouda F, Mohieddin O
Acta Biol Acad Sci Hung. 1977;28(3):291-8.
A histochemical technique for demonstrating leucocyte alkaline phosphatase activity (LAP), based on direct precipitation of lead phosphate at pH 9.5, is described. The effect of fixative, temperature and stability of the medium on the activity of the enzyme and stability of the colour reaction was thoroughly studied. Peripheral blood smears obtained from both normal humans and pathological cases were studied and the results were compared with these obtained by the azo-dye method.
本文描述了一种基于在pH 9.5条件下直接沉淀磷酸铅来显示白细胞碱性磷酸酶活性（LAP）的组织化学技术。深入研究了固定剂、温度和介质稳定性对酶活性及显色反应稳定性的影响。对从正常人和病理病例中获取的外周血涂片进行了研究，并将结果与偶氮染料法所得结果进行了比较。
