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手工琼脂扩散(鲍尔)技术与“阿巴克”半自动装置用于测定细菌“体外”抗生素敏感性的比较(作者译)

[Comparison of manual agar-diffusion (Bauer) technique and "Abac" semiautomatic device for the determination of "in vitro" bacterial sensitivity to antibiotics (author's transl)].

作者信息

Pagano A, Mascheroni E, Gualterotti S

出版信息

Ann Sclavo. 1979 Nov-Dec;21(6):790-9.

PMID:122055
Abstract

A comparison of antibiotic in vitro sensitivity tests performed on 115 freshly isolated Gram-negative bacterial strains with the manual standardized agar-diffusion technique according to Bauer and with the semiautomatic Abac (Intertechnique) device showed univocal results 85.5% of times, being Abac sensitivity 85.6% and specificity 86.2%. The frequency of univocal results is higher that in most of the previous trials, especially for ampicillin and cephalotin. No particular responses have been shown by the dirrerent microorganisms tested. After a discussion of the variables which can influence the results and the suggestion of some steps to be taken, the AA. analyze the role of Abac and its possible achievements in small and medium-size bacteriological laboratories.

摘要

采用鲍尔手动标准化琼脂扩散技术和半自动Abac(Intertechnique)设备,对115株新分离的革兰氏阴性菌进行抗生素体外敏感性试验比较,结果显示85.5%的情况下结果一致,Abac的敏感性为85.6%,特异性为86.2%。结果一致的频率高于大多数先前试验,尤其是氨苄西林和头孢噻吩。所测试的不同微生物未表现出特殊反应。在讨论了可能影响结果的变量并提出一些应采取的措施后,作者分析了Abac在中小型细菌学实验室中的作用及其可能取得的成果。

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