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从溶解成分中重建猪淋巴细胞质膜，特别涉及膜相关免疫球蛋白。

Reconstitution of pig lymphocyte plasma membranes from solubilized components, with particular reference to membrane-associated immunoglobulins.

作者信息

Chavin S I, Holliman A

出版信息

Biochem J. 1975 Nov;152(2):267-70. doi: 10.1042/bj1520267.

DOI:10.1042/bj1520267
PMID:1220684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1172468/
Abstract

Plasma membranes of lymphocytes obtained from pig mesenteric lymph nodes were reconstituted after solubilization with bile salts. The proportion by weight of immunoglobulin in the reconstituted membrane was no greater than about 5-10% of that in the original membrane. Possible reasons for the low reincorporation of immunoglobulin are discussed.

摘要

用胆汁盐溶解后，对从猪肠系膜淋巴结获取的淋巴细胞质膜进行了重构。重构膜中免疫球蛋白的重量比例不超过原始膜中免疫球蛋白重量比例的约5 - 10%。文中讨论了免疫球蛋白再掺入率低的可能原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab88/1172468/8f683bc2e7ab/biochemj00545-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab88/1172468/afa49c13e317/biochemj00545-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab88/1172468/8f683bc2e7ab/biochemj00545-0121-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab88/1172468/afa49c13e317/biochemj00545-0120-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab88/1172468/8f683bc2e7ab/biochemj00545-0121-a.jpg

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引用本文的文献

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3
Isolation of plasma and nuclear membranes of thymocytes. II. Biochemical composition.胸腺细胞血浆膜和核膜的分离。II. 生化组成。

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Comparison of the effects of various detergents on antigen-antibody interaction.各种去污剂对抗抗原-抗体相互作用的影响比较。
FEBS Lett. 1972 May 1;22(2):210-212. doi: 10.1016/0014-5793(72)80047-4.
3
Interaction of immunoglobulins with liposomes.免疫球蛋白与脂质体的相互作用。
J Cell Biol. 1978 Apr;77(1):232-45. doi: 10.1083/jcb.77.1.232.
J Clin Invest. 1974 Feb;53(2):536-43. doi: 10.1172/JCI107587.
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Native and reformed Mycoplasma laidlawii membranes compared by freeze-etching.
Biochim Biophys Acta. 1970;219(1):123-30. doi: 10.1016/0005-2736(70)90067-2.
5
Structural comparisons of native and reaggregated membranes from Mycoplasma laidawii and erythrocytes using a fluorescence probe.
Biochim Biophys Acta. 1971 Aug 13;241(2):422-30. doi: 10.1016/0005-2736(71)90042-3.
6
Plasma membrane protein subunit composition. A comparative study by discontinuous electrophoresis in sodium dodecyl sulfate.质膜蛋白亚基组成。通过十二烷基硫酸钠不连续电泳进行的比较研究。
J Biol Chem. 1971 Oct 25;246(20):6335-8.
7
[Partial reconstitution of an excitable membrane after dissolution by sodium deoxycholate].[经脱氧胆酸钠溶解后可兴奋膜的部分重建]
C R Acad Hebd Seances Acad Sci D. 1972 Jan 3;274(1):122-5.
8
Molecular biology of cellular membranes with applications to immunology.细胞膜的分子生物学及其在免疫学中的应用
Adv Immunol. 1974;19(0):1-66. doi: 10.1016/s0065-2776(08)60251-5.
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Reconstruction of biological membranes.生物膜的重建
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Binding of proteins to mycoplasma membranes.蛋白质与支原体膜的结合。
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