Ternes Waldemar, Krause Edburga L
School of Veterinary Medicine, Centre for Food Science, Department of Chemical Analytics, Bischofsholer Damm 15, 30173 Hannover, Germany.
Anal Bioanal Chem. 2002 Sep;374(1):155-60. doi: 10.1007/s00216-002-1416-6. Epub 2002 Aug 10.
A new analytical method for the determination of piperine and its isomers in egg yolk and albumen is described here. All four isomers were separated by HPLC and detected using UV, DAD and electrochemical detection. The absolute detection limit (UV detection, S/ N=3) of a standard solution of piperine was 370 pg piperine. The correlation coefficients for the linear calibration graphs (concentration range: c=100 ng-10 micro g piperine isomer/mL) are generally better than 0.996. The piperine isomers were characterized and identified by spectroscopy (MS, (1)H-NMR, FT-IR). The method was successfully applied to the determination of piperine deposits in eggs (egg yolk and albumen) after feeding hens with piperine-spiked feed. The detection limit for piperine (24.8(+/-0.2) ng/g egg yolk and 37.9(+/-4.9) ng/g albumen) and the recoveries (70.3(+/-7.7)% (egg yolk) and 75.7(+/-1.9)% (albumen)) of piperine were determined.
本文介绍了一种测定蛋黄和蛋清中胡椒碱及其异构体的新分析方法。通过高效液相色谱法分离所有四种异构体，并使用紫外、二极管阵列和电化学检测进行检测。胡椒碱标准溶液的绝对检测限（紫外检测，S/N = 3）为370皮克胡椒碱。线性校准曲线（浓度范围：c = 100纳克 - 10微克胡椒碱异构体/毫升）的相关系数通常优于0.996。通过光谱法（质谱、氢核磁共振、傅里叶变换红外光谱）对胡椒碱异构体进行了表征和鉴定。该方法成功应用于给母鸡喂食添加胡椒碱的饲料后鸡蛋（蛋黄和蛋清）中胡椒碱沉积量的测定。测定了胡椒碱的检测限（蛋黄中为24.8（±0.2）纳克/克，蛋清中为37.9（±4.9）纳克/克）以及回收率（蛋黄中为70.3（±7.7）%，蛋清中为75.7（±1.9）%）。
