Chang Sarah Y, Chiang Hsien-Tsung
Department of Applied Chemistry, Chaoyang University of Technology, Wufeng, Taichung County, Taiwan.
Electrophoresis. 2002 Sep;23(17):2913-7. doi: 10.1002/1522-2683(200209)23:17<2913::AID-ELPS2913>3.0.CO;2-5.
Capillary electrophoresis (CE) with indirect fluorescence detection was used to analyze selenium (selenite, selenate, selenomethionine, and selenocystine) and antimony (antimonite and antimonate) compounds. The separation was achieved by CE in 6 min with a 1.2 mM fluorescein solution at pH 9.5. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent species. Linearity of more than two orders of magnitude was generally obtained. Precision of migration times and peak areas was less than 1.0% and 7.2%, respectively. The concentration limits of detection (CLODs) was in the microM range. The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each species.
采用间接荧光检测的毛细管电泳（CE）法分析硒（亚硒酸盐、硒酸盐、硒代蛋氨酸和硒代胱氨酸）和锑（亚锑酸盐和锑酸盐）化合物。通过CE在6分钟内实现分离，使用pH 9.5的1.2 mM荧光素溶液。荧光素还作为背景荧光团用于间接检测这些非荧光物质。通常可获得超过两个数量级的线性关系。迁移时间和峰面积的精密度分别小于1.0%和7.2%。检测浓度限（CLODs）在微摩尔范围内。检测灵敏度通常取决于每种物质的转移比（TR，定义为一摩尔分析物离子取代的荧光素离子摩尔数）。
