Huang Y M, Whang C W
Department of Chemistry, Tunghai University, Taichung, Taiwan.
Electrophoresis. 1998 Sep;19(12):2140-4. doi: 10.1002/elps.1150191217.
A capillary electrophoresis (CE)-indirect fluorescence detection method for arsenic compounds is described. The five arsenic species, viz., arsenite (As(III)), arsenate (As(V)), monomethylarsonate (MMA), dimethylarsinate (DMA) and phenylarsonate (PhA), were efficiently separated by CE in 8 min with an 1.5 mM fluorescein solution at pH 9.8. Fluorescein also functioned as a background fluorophore for the indirect detection of these nonfluorescent arsenic species. Linearity (r> or =0.996) of more than two orders of magnitude was generally obtained. The relative standard deviation (RSD) values were in the ranges 0.4-0.7% and 2.2-8.2% for migration times and peak areas, respectively. The concentration limits of detection (CLODs) for the arsenic compounds studied were between 0.04 and 0.16 microg/mL (as arsenic). The detection sensitivity was generally dependent upon the transfer ratio (TR, defined as the number of moles of fluorescein ions displaced by one mole of analyte ions) of each arsenic species. The applicability of the method for the analysis of ground water was examined.
描述了一种用于砷化合物的毛细管电泳（CE）间接荧光检测方法。通过CE在8分钟内，使用pH 9.8的1.5 mM荧光素溶液，有效分离了五种砷物种，即亚砷酸盐（As(III)）、砷酸盐（As(V)）、一甲基砷酸盐（MMA）、二甲基砷酸盐（DMA）和苯砷酸盐（PhA）。荧光素还作为背景荧光团用于间接检测这些非荧光砷物种。通常获得了超过两个数量级的线性（r≥0.996）。迁移时间和峰面积的相对标准偏差（RSD）值分别在0.4 - 0.7%和2.2 - 8.2%范围内。所研究的砷化合物的检测浓度限（CLODs）在0.04至0.16 μg/mL（以砷计）之间。检测灵敏度通常取决于每种砷物种的转移比（TR，定义为一摩尔分析物离子取代的荧光素离子摩尔数）。考察了该方法在地下水分析中的适用性。
