Preparation and analysis of small unilamellar phospholipid vesicles of a uniform size.
作者信息
Pitcher Wayne H, Huestis Wray H
机构信息
Department of Chemistry, Stanford University, 94305, Stanford, CA, USA.
出版信息
Biochem Biophys Res Commun. 2002 Sep 6;296(5):1352-5. doi: 10.1016/s0006-291x(02)02092-2.
Abstract
The interaction of carbonmonoxyhemoglobin and heme with small unilamellar phospholipid vesicles was studied using dynamic light scattering. Addition of carbonmonoxyhemoglobin to dimyristoylphosphatidylcholine:dimyristoylphosphatidylserine small unilamellar vesicles resulted in an increase of average vesicle size from 17.4 to 32.0nm. Addition of heme to vesicles produced a smaller size increase, from 17.4 to 21.0nm. Also reported is a method for preparing small unilamellar lipid vesicles of a uniform size, suitable for use in NMR spectroscopy.
摘要
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