蒲黄质量标准研究
[Studies on quality standards for Pollen Typhae(puhuang)].
作者信息
Zhang S H, Wang S Q, Liu C H, Yang Y H
机构信息
Hunan Academy of Traditional Chinese Medicine and Materia Medica, Changsha 410006, Hunan, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2000 Mar;25(3):136-9.
OBJECTIVE
To work out quality standards for Pollen Typhae(Puhuang).
METHOD
Isorhamnetin-3-O-neohesperidoside, typhaneoside, isorhamnetin and beta-sitosterol in Pollen Typhae marketed in ten cities of China were identified by TLC. The contents of isorhamnetin-3-O-neohesperidoside and typhaneoside were determined by HPLC.
RESULT
The method is sensitive and specific for identifying isorhamnetin-3-O-neohesperidoside, typhaneoside, isorhamnetin and beta-sitosterol. The linear ranges of isorhamnetin-3-O-neohesperidoside and typhaneoside were 0.188-0.940 microgram with and average recovery of 97.77%; and 0.164-0.820 microgram with an average recovery of 98.42% respectively.
CONCLUSION
The method can be used both qualitatively and quantitatively as standards for the quality control of Pollen Typhae.
目的
制定蒲黄的质量标准。
方法
采用薄层色谱法（TLC）对中国十个城市销售的蒲黄中的异鼠李素-3-O-新橙皮糖苷、异鼠李素、香蒲新苷和β-谷甾醇进行鉴别。采用高效液相色谱法（HPLC）测定异鼠李素-3-O-新橙皮糖苷和香蒲新苷的含量。
结果
该方法对异鼠李素-3-O-新橙皮糖苷、异鼠李素、香蒲新苷和β-谷甾醇的鉴别灵敏且专属性强。异鼠李素-3-O-新橙皮糖苷和香蒲新苷的线性范围分别为0.188 - 0.940微克，平均回收率为97.77%；以及0.164 - 0.820微克，平均回收率为98.42%。
结论
该方法可用于蒲黄质量控制的定性和定量标准。
Zotero 插件