采用液相色谱/电喷雾串联质谱法分析大鼠血浆中的异鼠李素-3-O-新橙皮糖苷及其在药代动力学研究中的应用。
Analysis of isorhamnetin-3-O-neohesperidoside in rat plasma by liquid chromatography/electrospray ionization tandem mass spectrometry and its application to pharmacokinetic studies.
机构信息
Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.
Nanjing University of Traditional Chinese Medicine, Nanjing 210029, China.
出版信息
Chin J Nat Med. 2013 Sep;11(5):572-6. doi: 10.1016/S1875-5364(13)60103-X.
AIM
To establish an LC-MS/MS method for determination of isorhamnetin-3-O-neohesperidoside and investigate its application on pharmacokinetic study in rats.
METHODS
Eight rats were given 5 mg·kg(-1) isorhamnetin-3-O-neohesperidoside after intravenous administration. A highly sensitive liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of isorhamnetin-3-O-neohesperidosidein rat plasma using rutin as internal standard. The analytes and rutin (internal standard) were extracted with methanol followed by a rapid isocratic elution with 10 mmol·L(-1) ammonium acetate buffer/methanol (20 : 80, V/V) on a C18 column (150 mm × 2.1 mm, I.D., 5 μm) and subsequent analysis by mass spectrometry in the multi-eaction-monitoring mode.
RESULTS
The assays were linear over the concentration range of 0.01-10 μg·mL(-1) for isorhamnetin-3-O-neohesperidosidein rat plasma. The lower limit of quantifications for isorhamnetin-3-O-neohesperidoside was 0.01 μg·mL(-1).
CONCLUSION
The validated method is successfully applied to determine the plasma concentrations of isorhamnetin-3-O-neohesperidosidein in rats.
目的
建立 LC-MS/MS 法测定异槲皮苷-3-O-新橙皮苷，并考察其在大鼠体内药代动力学研究中的应用。
方法
8 只大鼠静脉给予 5mg·kg(-1)异槲皮苷-3-O-新橙皮苷后，采用高效液相色谱-串联质谱法，以芦丁为内标，测定大鼠血浆中异槲皮苷-3-O-新橙皮苷的浓度。用甲醇提取，采用 10mmol·L(-1)乙酸铵缓冲液-甲醇（20：80，V/V）等度洗脱，在 C18 柱（150mm×2.1mm，内径，5μm）上进行分析，采用多反应监测模式进行质谱检测。
结果
异槲皮苷-3-O-新橙皮苷在大鼠血浆中的浓度在 0.01-10μg·mL(-1)范围内呈线性关系。异槲皮苷-3-O-新橙皮苷的定量下限为 0.01μg·mL(-1)。
结论
该方法已成功应用于测定大鼠血浆中异槲皮苷-3-O-新橙皮苷的浓度。
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